Development of a molecular marker for sex identification of <i>Spodoptera frugiperda</i>

LIN Jinbei; WEI Jiaqi; WANG Jiali; ZHU Jian; LI Mao; XU Hanhong; LIN Fei

doi:10.7671/j.issn.1001-411X.202012033

Journal of South China Agricultural University > 2021 > 42(4): 70-74. > DOI: 10.7671/j.issn.1001-411X.202012033

LIN Jinbei, WEI Jiaqi, WANG Jiali, et al. Development of a molecular marker for sex identification of Spodoptera frugiperda[J]. Journal of South China Agricultural University, 2021, 42(4): 70-74. DOI: 10.7671/j.issn.1001-411X.202012033

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Development of a molecular marker for sex identification of Spodoptera frugiperda

College of Plant Protection, South China Agricultural University/Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642, China

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Received Date: December 28, 2020
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To develop molecular marker primers for sexual identification of Spodoptera frugiperda.

Method
Based on the difference in pheromone-binding protein (PBP) gene sequences of male and female S. frugiperda, the primers were designed for distinguishing sexual distinction specifically. Using the primers, the male and female S. frugiperda samples already identified by morphology were further verified by PCR.

Result
Specific bands of about 450 bp for the corresponding female and male samples were amplified by two pairs of primers respectively.

Conclusion
The molecular marker primers can be used to discriminate the sex of S. frugiperda in various developmental stages efficiently, and provide an effective tool for the study of the individual distinction between different sexual insects. This study provide a reference for formulating the strategy to control S. frugiperda effectively based on sexual distinction.
- Spodoptera frugiperda,
- pheromone-binding protein,
- sex identification,
- molecular marker primer

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References (24)

References

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Development of a molecular marker for sex identification of Spodoptera frugiperda