Pathogen identification of anthracnose disease on <i>Millettia speciosa</i> and indoor determination of fungicide toxicity

XU Dandan; SHI Liyun; LIN Zemian; LI Yiting; JIANG Zide; QIAO Fang

doi:10.7671/j.issn.1001-411X.202012008

Journal of South China Agricultural University > 2021 > 42(4): 63-69. > DOI: 10.7671/j.issn.1001-411X.202012008

XU Dandan, SHI Liyun, LIN Zemian, et al. Pathogen identification of anthracnose disease on Millettia speciosa and indoor determination of fungicide toxicity[J]. Journal of South China Agricultural University, 2021, 42(4): 63-69. DOI: 10.7671/j.issn.1001-411X.202012008

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Pathogen identification of anthracnose disease on Millettia speciosa and indoor determination of fungicide toxicity

1.
Department of Applied Chemistry and Biotechnology/Postdoctoral Innovation Practice Base, Shenzhen Polytechnic, Shenzhen 518055, China
2.
College of Plant Protection, South China Agricultural University/Guangdong Provincial Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, China

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Received Date: December 02, 2020
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To determine the pathogen causing anthracnose disease on Millettia speciosa and screen effective fungicides.

Method
Tissue isolation method was used to isolate the pathogen. The pathogenicity was tested according to Koch’s rule after purification. The pathogen was identified based on morphological characteristics and multi-locus sequence analysis. Furthermore, the sensitivity of this pathogen against four common fungicides was measured according to the mycelial growth rate.

Result
Two out of six obtained isolates could infect M. speciosa leaf and caused brown spot. Combining morphological characteristics and multi-locus sequences analysis, the pathogen caused anthracnose disease on M. speciosa was identified as Colletotrichum siamense. This pathogen was highly sensitive to difenoconazole, prochloraz, pyraclostrobin and thiophanate-methyl with EC₅₀ values below 0.1 mg/L, and prochloraz showed the highest efficacy with EC₅₀ of 0.015 mg/L.

Conclusion
The pathogen causing anthracnose disease on M. speciosa is C. siamense, and difenoconazole, prochloraz, pyraclostrobin and thiophanate-methyl can be applied to control anthracnose disease in the field.

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References (29)

References

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