Identification of autophagy-related genes in fall armyworm, <i>Spodoptera frugiperda</i>

MA Qiuqin; WANG Yusheng; LIANG Kuan; HAN Yong; YI Huiyu; DENG Xiaojuan

doi:10.7671/j.issn.1001-411X.202004007

Journal of South China Agricultural University > 2021 > 42(1): 93-100. > DOI: 10.7671/j.issn.1001-411X.202004007

MA Qiuqin, WANG Yusheng, LIANG Kuan, et al. Identification of autophagy-related genes in fall armyworm, Spodoptera frugiperda[J]. Journal of South China Agricultural University, 2021, 42(1): 93-100. DOI: 10.7671/j.issn.1001-411X.202004007

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Identification of autophagy-related genes in fall armyworm, Spodoptera frugiperda

College of Animal Science, South China Agricultural University/Key Laboratory of Agro-animal Genomics and Molecular Breeding of Guangdong Province/Guangdong Provincial Sericulture and Mulberry Engineering Research Center, Guangzhou 510642, China

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Received Date: April 02, 2020
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To identify autophagy-related genes (Atg) in fall armyworm (Spodoptera frugiperda) genome and their response to viral infection in S. frugiperda, and provide a basis for further research on the molecular mechanism of autophagy.

Method
Autophagy-related protein (ATG) sequences from various species, such as human (Homo sapiens) and fruit fly (Drosophila melanogaster), were aligned and analyzed for sequence similarity. ATG sequences in S. frugiperda genome were searched. Conservative domain (CD) of the candidate ATG were analyzed by NCBI CD search. Furthermore, qRT-PCR was used to detect the expression changes of Atgs in Sf9 cells after infection by recombinant EGFP-AcMNPV.

Result
We identified more than ten core Atgs which were involved in autophagosome formation in S. frugiperda, including the complete sequences of Atg3, Atg5, Atg6, Atg7, Atg10, Atg12, Atg13 and Atg101, and the partial sequences of Atg1, Atg2, Atg4, Atg9, Atg14, Atg16, Atg17 and Atg18. The expression levels of S. frugiperda Atg2, Atg4, Atg5, Atg6, Atg7, Atg8 and Atg12 were up-regulated after 6 to 12 hours infection by EGFP-AcMNPV.

Conclusion
Eukaryotic conserved Atgs exist in the genome of S. frugiperda, indicating the autophagy pathway in S. frugiperda is evolutionarily conserved. AcMNPV infection might trigger the autophagic response in Sf9 cells.
- Spodoptera frugiperda,
- autophagy,
- autophagy-related protein,
- conservative domain,
- autophagy pathway

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References (26)

References

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