Biodiversity comparison of endophytic fungi from wild and artificially cultivated <i>Dendrobium officinale</i> and screening of fungal strains with anti-anthracnose activities

LIU Yanming; HUANG Yali; LI Ting; FENG Meiru; SHI Wenguang; DENG Zujun

doi:10.7671/j.issn.1001-411X.202001034

Journal of South China Agricultural University > 2020 > 41(3): 47-55. > DOI: 10.7671/j.issn.1001-411X.202001034

LIU Yanming, HUANG Yali, LI Ting, et al. Biodiversity comparison of endophytic fungi from wild and artificially cultivated Dendrobium officinale and screening of fungal strains with anti-anthracnose activities[J]. Journal of South China Agricultural University, 2020, 41(3): 47-55. DOI: 10.7671/j.issn.1001-411X.202001034

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Biodiversity comparison of endophytic fungi from wild and artificially cultivated Dendrobium officinale and screening of fungal strains with anti-anthracnose activities

1.
College of Life Science and Biopharmaceutics, Guangdong Pharmaceutical University/Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou 510006, China
2.
School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

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Received Date: January 20, 2020
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To systematically analyze and compare the diversity of endophytic fungi from wild and cultivated Dendrobium officinale, and screen fungal strains with anti-anthracnose activities.

Method
Endophytic fungi of D. officinale were isolated by tissue block separation method, and they were identified by slide culture method and molecular identification method based on internal transcribed spacer regions of ribosomal DNA (rDNA-ITS). Screening for endophytic fungi against anthracnose was carried out by plate method.

Result
Totally 236 endophytic fungal strains were isolated from D. officinale(132 from wild plants and 104 from artificially cultivated plants). Total colonization rate (50.46%), total isolation rate (61.11%) and diversity index (2.95%) of endophytic fungi in wild plants were significantly higher than those in artificially cultivated plants (the corresponding values were 39.35%, 48.14% and 2.49, respectively). We screened out 34 strains of endophytic fungi with inhibitory activities against Colletotrichum gloeosporioides. Amomg them, the strains Ceriporia sp. F102, Rigidoporus sp. F27, Termitomyces sp. F103 and Peniophora sp. F122 showed higher inhibitory effects, and their inhibition rates were 93.06%, 88.20%, 87.48% and 84.57%, respectively.

Conclusion
The biodiversity and community structure of endophytic fungi of wild D. officinale and artificially cultivated D. officinal had significant differences, and abundant strains with anti-anthracnose activities were obtained from them, which lays the foundation for improving the quality of medicinal materials of artificially cultivated D. officinal and the biocontrol of D. officinale anthracnose.
- Dendrobium officinale,
- endophytic fungus,
- biodiversity,
- anthracnose,
- Colletotrichum gloeosporioides

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Biodiversity comparison of endophytic fungi from wild and artificially cultivated Dendrobium officinale and screening of fungal strains with anti-anthracnose activities

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