Effects of chemical substances on melanin formation of <i>Rhizoctonia solani</i> AG-1 IA

JIANG Shaofeng; SHI Yunjing; ZHAO Mei; SHU Canwei; ZHOU Erxun

doi:10.7671/j.issn.1001-411X.201911010

Journal of South China Agricultural University > 2020 > 41(4): 49-56. > DOI: 10.7671/j.issn.1001-411X.201911010

JIANG Shaofeng, SHI Yunjing, ZHAO Mei, et al. Effects of chemical substances on melanin formation of Rhizoctonia solani AG-1 IA[J]. Journal of South China Agricultural University, 2020, 41(4): 49-56. DOI: 10.7671/j.issn.1001-411X.201911010

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Effects of chemical substances on melanin formation of Rhizoctonia solani AG-1 IA

1.
College of Agriculture, South China Agricultural University/Guangdong Provincial Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, China
2.
School of Basic Medical Sciences, Guilin Medical University, Guilin 541000, China

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Received Date: November 05, 2019
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
Rice sheath blight, caused by Rhizoctonia solaniAG-1 IA, is an important fungal disease. The purpose of this study was to determine the relationship between external culture conditions and melanin formation of R. solani AG-1 IA.

Method
The effects of four different treatment groups, i. e. chemical fertilizer group [500 μg/mL K₂SO₄, NaH₂PO₄, CO(NH₂)₂], metal ion compound group(5 μg/mL CuSO₄, FeSO₄, ZnSO₄), antioxidant group (5 μg/mL quercetin, morin, vitamin C) and control group (300 μg/mL hyoscyamine, 50 μg/mL catechol, water), on the mycelial growth rate, sclerotia number, sclerotial fresh weight and dry weight as well as melanin content of R. solani AG-1 IA were determined by measuring mycelial growth rate and ultraviolet spectrophotometry.

Result
Chemical fertilizer [500 μg/mL K₂SO₄, NaH₂PO₄, CO(NH₂)₂], metal ion compound (5 μg/mL CuSO₄, ZnSO₄), antioxidant(5 μg/mL quercetin, morin, vitamin C) and catechol(50 μg/mL) could promote the formation of melanin. Under the treatment of 500 μg/mL CO(NH₂)₂, the melanin content of R. solani AG-1 IA was the highest (113.2 mg), while under the treatment of 300 μg/mL hyoscyamine, the melanin content of R. solani AG-1 IA was the lowest (37.4 mg).

Conclusion
Melanin formation is not necessarily related to mycelial growth and sclerotial development, i.e. the chemical substances, which have inhibitory effect on mycelial growth and sclerotial development, might have no inhibition on melanin formation. The results of this study provide a basis for understanding the mechanism for prevention and control of rice sheath blight.
- Rhizoctonia solani AG-1 IA,
- mycelium,
- sclerotium,
- melanin,
- chemical substance

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References (26)

References

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