Antibacterial activity and mechanism of garlic organic sulfide against <i>Bacillus amyloliquefaciens</i>

DAI Boren; SHEN Guanghui; LIU Haina; ZHANG Lidan; HE Chunqiao; GE Runqiu

doi:10.7671/j.issn.1001-411X.201910018

Journal of South China Agricultural University > 2020 > 41(4): 95-103. > DOI: 10.7671/j.issn.1001-411X.201910018

DAI Boren, SHEN Guanghui, LIU Haina, et al. Antibacterial activity and mechanism of garlic organic sulfide against Bacillus amyloliquefaciens[J]. Journal of South China Agricultural University, 2020, 41(4): 95-103. DOI: 10.7671/j.issn.1001-411X.201910018

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Antibacterial activity and mechanism of garlic organic sulfide against Bacillus amyloliquefaciens

College of Food Science, Sichuan Agricultural University, Ya′an 625014, China

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Received Date: October 23, 2019
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To explore antibacterial activity and mechanism of garlic organic sulfide (GOS) against Bacillus amyloliquefaciens.

Method
The antimicrobial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of GOS against B. amyloliquefaciens strain were determined using the inhibition zone method and the double dilution method, and its inhibitory effects on bacterial growth curve and extracellular protease activity were evaluated. Metabolic activity, alkaline phosphatase activity, β-galactosidase activity, extracellular conductivity and thallus morphological changes observed through scanning electron microscope of B. amyloliquefaciens treated with GOS were investigated for elucidating antibacterial mechanism of GOS against B. amyloliquefaciens.

Result
Diameter of inhibitory zone of GOS against B. amyloliquefaciens DY1a and DY1b were 31.7 and 25.7 mm respectively, MIC of both strains were 30 mg/mL, MBC were 240 and 480 mg/mL, respectively. The inhibition rate of 30 mg/mL GOS on DY1a and DY1b extracellular protease activity were 47.3% and 15.6% respectively. After treatment with 30 mg/mL GOS, metabolic activity (D_{490 nm}) decreased by 2.771 and 4.091 respectively, extracellular alkaline phosphatase activity increased by 0.029 and 0.036 U/mL, extracellular β-galactosidase activity (D_{420 nm}) increased by 0.047 and 0.016 respectively and the extracellular electrical conductivity increased by 0.060 and 0.031 mS/cm respectively. The results of SEM showed that the surface of bacteria cells treated with GOS had shrinkage, cavity collapse and rupture.

Conclusion
The excellent antibacterial effect of GOS against B. amyloliquefaciens may be attributed to irreversible damage to cell wall and cell membrane and alterations of cell physiological metabolism.
- Bacillus amyloliquefaciens,
- garlic organic sulfide,
- antibacterial activity,
- antibacterial mechanism

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References (25)

References

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Antibacterial activity and mechanism of garlic organic sulfide against Bacillus amyloliquefaciens

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