Virulence gene profiles and molecular typing of <i>Streptococcus iniae</i> isolated from sturgeon in Sichuan

BAI Minghuan; GENG Yi; ZHAO Ruoxuan; GUO Xianghui; HUANG Xiaoli; CHEN Defang; OUYANG Ping; WANG Jiao

doi:10.7671/j.issn.1001-411X.201910005

Journal of South China Agricultural University > 2020 > 41(5): 36-42. > DOI: 10.7671/j.issn.1001-411X.201910005

BAI Minghuan, GENG Yi, ZHAO Ruoxuan, et al. Virulence gene profiles and molecular typing of Streptococcus iniae isolated from sturgeon in Sichuan[J]. Journal of South China Agricultural University, 2020, 41(5): 36-42. DOI: 10.7671/j.issn.1001-411X.201910005

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Virulence gene profiles and molecular typing of Streptococcus iniae isolated from sturgeon in Sichuan

1.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China

More Information

Received Date: October 14, 2019
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To clarify the virulence gene profiles and molecular typing of Streptococcus iniae from sturgeon in Sichuan, and provide a reference for prevention and control of S. iniae infection in sturgeon.

Method
Virulence genes of 17 S. iniae strains isolated from sturgeon in Sichuan area and S. iniae ATCC29178 were detected by multi-PCR. Molecular typing of S. iniae was performed using the random amplified polymorphic DNA (RAPD) and repetitive element polymerase chain reaction (REP-PCR) methods.

Result
All seven major virulence genes includingpgm , scpI , simA , cpsD ,sagA ,pdi and cfi were positive in all strains, and simA gene of partial isolates was mutated. Eighteen strains were divided intoⅠandⅡ types based on RAPD analysis, and four types of A, B, C and D by REP-PCR analysis.

Conclusion
All 17 S. iniae isolates from sturgeon in Sichuan are virulent strains, and the virulence gene prolife is pgm/scpI/simA/cpsD/sagA/pdi/cfi. There are many genotypes of S. iniae in Sichuan, of which type D is the main epidemic type.
- sturgeon,
- Streptococcus iniae,
- virulence gene,
- molecular typing,
- RAPD,
- REP-PCR

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References (35)

References

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