Citation: | ZHANG Rong, XU Dandan, JIANG Liqun, et al. Effects of litchi pericarp polyphenols on biological characteristics of Peronophythora litchii[J]. Journal of South China Agricultural University, 2019, 40(6): 82-87. DOI: 10.7671/j.issn.1001-411X.201907003 |
To investigate the effects of litchi polyphenols on growth and development of Peronophythora litchii.
Polyphenols were extracted from litchi pericarp by solvent extraction. The effects of polyphenols on colony morphology, biomass, germination mode of sporangium, zoospore germination and oospore number of P. litchii were analyzed by phytopathological research methods.
With the treatment of litchi polyphenols at 150 to 300 μg·mL−1, the hyphal colonies of P. litchii appeared dense, hyphal dry weight and sporangia number were significantly reduced, and the sporangia germinated to release zoospores. With the treatment of litchi polyphenols at 100 to 300 μg·mL−1, zoospores germination was significantly increased while sporangia germination was significantly suppressed. Oospore was hardly observed in PDA control without litchi polyphenols. When litchi polyphenols in the media reached 100 μg·mL−1, the produce of oospores was 10.8 mm−2.
Litchi polyphenols involve in the regulation of the growth and development of P. litchii, can suppress the growth of P. litchii and sporangia germination, indicating their potential for developing new safe biogenic fungicides.
[1] |
张荣. 荔枝霜疫霉侵染过程研究及农业措施控制作用初探[D]. 广州: 华南农业大学, 2012: 25.
|
[2] |
李堃娟, 黄远峰, 叶建春, 等. 五种杀菌剂防治荔枝霜疫病的田间药效试验[J]. 广西农学报, 2013, 28(2): 12-13. doi: 10.3969/j.issn.1003-4374.2013.02.004
|
[3] |
易赛, 潘汝谦, 徐大高, 等. 荔枝霜疫霉菌对烯酰吗啉的敏感性测定[J]. 广东农业科学, 2014, 41(2): 87-91. doi: 10.3969/j.issn.1004-874X.2014.02.022
|
[4] |
MA Q, XIE H, JIANG Y, et al. Phenolics and sesquiterpenes from litchi pericarp[J]. J Funct Foods, 2014, 9: 156-161. doi: 10.1016/j.jff.2014.04.020
|
[5] |
IARAHIM S R M, MOHAMED G A. Litchi chinensis: Medicinal uses, phytochemistry, and pharmacology[J]. J Ethnopharmacol, 2015, 174: 492-513. doi: 10.1016/j.jep.2015.08.054
|
[6] |
WEN L, WU D, JIANG Y, et al. Identification of flavonoids in litchi (Litchi chinensis Sonn.) leaf and evaluation of anticancer activities[J]. J Funct Foods, 2014, 6: 555-563. doi: 10.1016/j.jff.2013.11.022
|
[7] |
XU X, XIE H, WANG Y, et al. A-type proanthocyanidins from lychee seeds and their antioxidant and antiviral activities[J]. J Agric Food Chem, 2010, 58: 11667-11672. doi: 10.1021/jf1033202
|
[8] |
WANG M, JIANG N, WANG Y, et al. Characterization of phenolic compounds from early and late ripening sweet cherries and their antioxidant and antifungal activities[J]. J Agric Food Chem, 2017, 65: 5413-5420. doi: 10.1021/acs.jafc.7b01409
|
[9] |
XU D, DENG Y, HAN T, et al. In vitro and in vivo effectiveness of phenolic compounds for the control of postharvest gray mold of table grapes[J]. Postharvest Biol Tec, 2018, 139: 106-114. doi: 10.1016/j.postharvbio.2017.08.019
|
[10] |
王敏, 陈磊, 黄雪松. 荔枝中多酚含量的测定[J]. 食品与发酵工业, 2010, 36(2): 172-175.
|
[11] |
SINGLETON V L, ROSSI J A. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents[J]. Am J Enol Viticult, 1965, 16: 144-158.
|
[12] |
曾勇, 罗建军, 丘麒, 等. 23种植物提取物对荔枝霜疫霉病菌的抑菌活性[J]. 华中农业大学学报, 2007, 26(6): 780-784. doi: 10.3321/j.issn:1000-2421.2007.06.006
|
[13] |
方中达. 植病研究方法[M]. 北京: 中国农业出版社, 1998: 40-41.
|
[14] |
KO W H, CHASE L L, KUNIMOTO R K. A microsyringe method for determining concentration of fungal propagules[J]. Phytopathol, 1973, 63: 1206-1207. doi: 10.1094/Phyto-63-1206
|
[15] |
KAO W H, LEU L S. Sporangium germination of Peronophythora litchii, the causal organism of litchi downy blight[J]. Mycologia, 1980, 72(4): 737-748. doi: 10.1080/00275514.1980.12021242
|
[16] |
NEUFELD K N, OJIAMBO P S. Interactive effects of temperature and leaf wetness duration on sporangia germination and infection of cucurbit hosts by Pseudoperonospora cubensis[J]. Plant Dis, 2012, 96(3): 345-353. doi: 10.1094/PDIS-07-11-0560
|
[17] |
PAULINE B, GRANT B R. Some conditions governing zoospore production in axenic cultures of Phytophthora cinnamomi Rands[J]. Aust J Bot, 1979, 27(2): 103-115. doi: 10.1071/BT9790103
|
[18] |
FLIER W G, GRÜNWALD N J, FRY W E, et al. Formation, production and viability of oospores of Phytophthora infestans from potato and Solanum demissum in the Toluca Valley, central Mexico[J]. Mycol Res, 2001, 105(8): 998-1006. doi: 10.1016/S0953-7562(08)61958-9
|
[19] |
黄谦. 蓝药睡莲(Nymphaea stellata Willd)多酚活性研究及遗传毒性评价[D]. 成都: 四川师范大学, 2009.
|
[20] |
LAMBERT C, BISSO J, WAFFO-TEGUO P, et al. Phenolics and their antifungal role in grapevine wood decay: Focus on the Botryosphaeriaceae family[J]. J Agric Food Chem, 2012, 60: 11859-11868.
|