Sensitivity of four phages to host bacterium <i>Ralstonia solanacearum</i> and analysis of their receptors

YU Chengpeng; HU Ronghua; LIN Peijiong; LI Xingwei; ZHONG Min; LIU Qiongguang

doi:10.7671/j.issn.1001-411X.201903022

Journal of South China Agricultural University > 2020 > 41(2): 82-87. > DOI: 10.7671/j.issn.1001-411X.201903022

YU Chengpeng, HU Ronghua, LIN Peijiong, et al. Sensitivity of four phages to host bacterium Ralstonia solanacearum and analysis of their receptors[J]. Journal of South China Agricultural University, 2020, 41(2): 82-87. DOI: 10.7671/j.issn.1001-411X.201903022

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Sensitivity of four phages to host bacterium Ralstonia solanacearum and analysis of their receptors

1.
College of Agriculture, South China Agricultural University, Guangzhou 510642, China
2.
Ji'an Tobacco Company, Ji′an 343000, China
3.
Key Laboratory of Microbial Signals and Disease Control of Guangdong Province, Guangzhou 510642, China

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Received Date: April 15, 2019
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the lysis abilities of different phages mixtures against Ralstonia solanacearum, study their effects on the generation of phage-resistant strains, and clarify the receptors of four types of phages.

Method
Four types of phages, including P1556-1, P1556-2, P7-1 and P1521, were mixed in pairs. The sizes of plaques on R. solanacearum plates were determined. The resistance of R. solanacearum to phages was measured by co-culture of R. solanacearum and phages. Adsorption tests using phages, lipopolysaccharide (LPS) and membrane protein were conducted to determine the receptors of phages.

Result
Phage P1556-1 produced the largest plaque, indicating a strong lytic ability against R. solanacearum. The plaque sizes from the mixtures of two types of phages did not significantly differ from those of individual phages, while mixing two types of phages could slow down the generation of phage-resistant strains. Phage P1556-2 could be adsorbed by LPS of R. solanacearum, phage P1521 could be adsorbed by both LPS and membrane protein, while phage P1556-1 and P7-1 could be adsorbed by neither LPS nor membrane protein.

Conclusion
Mixing different phages can not improve the lysis ability against R. solanacearum, but can delay the generation of resistant host strain. The receptors of different phages are different.
- Ralstonia solanacearum,
- lytic phage,
- host resistance,
- receptor,
- plaque

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References (27)

References

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Sensitivity of four phages to host bacterium Ralstonia solanacearum and analysis of their receptors

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