Identification of two fungi strains and their control effects to cereal cyst nematode

CHEN Xiuju; JIAN Jinzhuo; LI Huixia; LI Rui; LU Zhiqin

doi:10.7671/j.issn.1001-411X.201903015

Journal of South China Agricultural University > 2020 > 41(1): 108-115. > DOI: 10.7671/j.issn.1001-411X.201903015

CHEN Xiuju, JIAN Jinzhuo, LI Huixia, et al. Identification of two fungi strains and their control effects to cereal cyst nematode[J]. Journal of South China Agricultural University, 2020, 41(1): 108-115. DOI: 10.7671/j.issn.1001-411X.201903015

Citation:

PDF (992 KB)

Identification of two fungi strains and their control effects to cereal cyst nematode

College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China

More Information

Received Date: March 10, 2019
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To identify the species of biocontrol strains and analyze their control efficiency to cereal cyst nematodes (Heterodera avenae).

Method
Biocontrol fungus A1 and B1 were identified according to morphological characters, rDNA-ITS sequence alignment and analysis of phylogenetic tree. The control effects of the fermentation broths of strain A1 and B1 against cysts, eggs and the second-stage juveniles (J2) and pot control effects against cereal cyst nematodes were determined.

Result
Strain A1 and B1 were identified as Aspergillus parasiticus and Trichoderm longilbrachiatum respectively. The mycelium parasitic rates of A1 and B1 on nematode cysts were over 80.00% at 14 days after treatment. At 12 days after treatment, the inhibition rates of the fermentation broths of A1 and B1 on egg hatching were 63.98 % and 67.56% respectively. The corrected mortality rates of J2 at 72 hours after treatment were 68.14% and 92.98 % respectively. At 90 days after treatment with 3% fungus fertilizer, pot experiment results showed that cyst reduction rates of A1 and B1 were 31.71% and 36.04%, the root lengths of wheat plants increased by 28.82% and 59.62%, and the plant heights increased by 20.38% and 21.43%, respectively.

Conclusion
Strain A1 and B1 have control effects on cereal cyst nematodes and are worthy of development and utilization .
- Aspergillus parasiticus,
- Trichoderma longilbrachiatum,
- Heterodera avenae,
- control effect,
- biocontrol strain

FullText(HTML)

References (38)

References

[1]	刘维志. 植物线虫志[M]. 北京: 中国农业出版社, 2004.
[2]	NICOL J M, RIVOAL R. Global knowledge and its application for the integrated control and management of nematodes on wheat[M]//CIANCIO A, MUKERJI K G. Integrated management and biocontrol of vegetable and grain crops nematodes, Dordrecht: Springer. 2008: 251-294.
[3]	张洁, 杨丽荣, 夏明聪, 等. 小麦孢囊线虫病综合防治研究进展[J]. 河南农业科学, 2017(5): 8-14.
[4]	李红梅, 王暄, 彭德良. 小麦孢囊线虫病概况及江苏省的发生现状与防治对策[J]. 江苏农业科学, 2010(6): 1-4. doi: 10.3969/j.issn.1002-1302.2010.06.001
[5]	王明祖, 彭德良. 小麦孢囊线虫病的研究: I: 病原鉴定[J]. 华中农业大学学报, 1991, 10(4): 352-356.
[6]	王振跃, 王守正, 李洪连, 等. 河南省小麦孢囊线虫病的初步研究[J]. 华北农学报, 1993, 8(S1): 105.
[7]	刘维志, 刘修勇, 栾兆杰. 山东省菏泽市郊麦田发现燕麦胞囊线虫(Heterodera avenae)[J]. 青岛农业大学学报(自然科学版), 2005, 22(4): 266-269. doi: 10.3969/j.issn.1674-148X.2005.04.006
[8]	郑经武, 林茂松, 程瑚瑞, 等. 安徽省小麦上两个孢囊线虫群体的鉴定[J]. 江苏农业学报, 1996(1): 31-35.
[9]	坚晋卓. 禾谷孢囊线虫生防菌株的筛选及鉴定[D]. 兰州: 甘肃农业大学, 2016.
[10]	李惠霞, 刘永刚, 朱锐东, 等. 甘肃省小麦禾谷孢囊线虫的发生及分布[J]. 植物保护, 2016(3): 170-174. doi: 10.3969/j.issn.0529-1542.2016.03.030
[11]	刘静, 吴海燕, 彭德良. 小麦孢囊线虫的发生防治及控制策略的思考[J]. 安徽农业科学, 2010, 38(3): 1629-1632. doi: 10.3969/j.issn.0517-6611.2010.03.080
[12]	刘刚. 农业部种植业管理司提醒: 跨区收割可传带小麦孢囊线虫[J]. 农药市场信息, 2009(16): 42.
[13]	黄忠勤, 王波, 周兴根, 等. 小麦孢囊线虫病害发生规律、鉴定方法及防控策略综述[J]. 麦类作物学报, 2013, 33(1): 200-206. doi: 10.7606/j.issn.1009-1041.2013.01.035
[14]	GRONVOLD J, HENRIKSEN S A, LARSEN M, et al. Biological control, aspects of biological control: With special reference to arthropods, protozoans and helminths of domesticated animals[J]. Vet Parasitol, 1996, 64(1/2): 47.
[15]	张洁. 小麦孢囊线虫病的生防微生物鉴定及球孢白僵菌08F04菌株对土壤微生物多样性的影响[D]. 郑州: 河南农业大学, 2016.
[16]	赵培静, 任文彬, 缪承杜, 等. 淡紫拟青霉研究进展与展望[J]. 安徽农业科学, 2007, 35(30): 9672-9674. doi: 10.3969/j.issn.0517-6611.2007.30.095
[17]	卢明科, 潘沧桑, 李舟. 厚垣轮枝孢菌(Verticillium chlamydosporium)防治植物线虫研究进展[J]. 西北农林科技大学学报(自然科学版), 2004, 32(4): 103-107. doi: 10.3321/j.issn:1671-9387.2004.04.025
[18]	孙漫红, 刘杏忠, 缪作清. 大豆胞囊线虫病生物防治研究进展[J]. 中国生物防治学报, 2000, 16(3): 136-141. doi: 10.3969/j.issn.2095-039X.2000.03.010
[19]	刘翠君, 曹春霞, 周荣华, 等. 苏云金杆菌防治大豆孢囊线虫效果试验[J]. 湖北农业科学, 2011, 50(3): 520-522. doi: 10.3969/j.issn.0439-8114.2011.03.027
[20]	ZHANG J, LI Y, YUAN H, et al. Biological control of the cereal cyst nematode (Heterodera filipjevi) by Achromobacter xylosoxidans isolate 09X01 and Bacillus cereus isolate 09B18[J]. Biol Control, 2016, 92: 1-6. doi: 10.1016/j.biocontrol.2015.08.004
[21]	ALESSANDRI A, KNAP I, ZAMBELLI L S D F. Nematicidal composition comprising Bacillus subtilis and Bacillus licheniformis: US, 9485993[P]. 2016-11-08.
[22]	代园凤, 张承, 喻会平, 等. 一株寄生曲霉SF-2对甘蔗绵蚜的感染作用及其生物学特性初步研究[J]. 云南农业大学学报(自然科学版), 2017, 32(6): 1006-1011.
[23]	姚粟, 李辉, 程池. 23株曲霉属菌种的形态学复核鉴定研究[J]. 食品与发酵工业, 2006, 32(12): 37-43. doi: 10.3321/j.issn:0253-990X.2006.12.009
[24]	坚晋卓, 徐鹏刚, 张虎忠, 等. 真菌AT9对禾谷孢囊线虫的寄生作用及种类鉴定[J]. 甘肃农业大学学报, 2016, 51(5): 71-77.
[25]	邹积雪, 季乃云. 松节藻来源长枝木霉DL5-4化学成分及其生物活性研究[J]. 化学与生物工程, 2019(3): 13-17. doi: 10.3969/j.issn.1672-5425.2019.03.003
[26]	张树武, 徐秉良, 薛应钰, 等. 长枝木霉T6菌株对黄瓜南方根结线虫的防治及其根际定殖作用[J]. 应用生态学报, 2016, 27(1): 250-254.
[27]	张树武, 徐秉良, 薛应钰, 等. 长枝木霉对禾谷胞囊线虫的寄生和致死作用[J]. 微生物学报, 2014, 54(7): 793-802.
[28]	田涛, 孙淑琴, 杨秀荣, 等. 植物病害生防细菌的筛选策略综述[J]. 天津农业科学, 2012, 18(5): 162-165. doi: 10.3969/j.issn.1006-6500.2012.05.042
[29]	ABAWI G S, WIDMER T L. Impact of soil health management practices on soilborne pathogens, nematodes and root diseases of vegetable crops[J]. Appl Soil Ecol, 2000, 15(1): 37-47. doi: 10.1016/S0929-1393(00)00070-6
[30]	迟君道, 王笑庸, 樊川, 等. 寒地大豆胞囊线虫生防菌的筛选及防治效果研究[J]. 黑龙江科学, 2015(5): 20-21. doi: 10.3969/j.issn.1674-8646.2015.05.008
[31]	李婷, 黄文坤, 彭德良, 等. 3株生防真菌发酵液对大豆孢囊线虫的防治效果[J]. 华中农业大学学报, 2017, 36(1): 42-46.
[32]	梁晨, 黄晨, 宋雯雯, 等. 菌株S01324对燕麦孢囊线虫的杀线活性及其种类鉴定[J]. 植物病理学报, 2018, 48(6): 838-846.
[33]	申佩娟, 苗利华, 李娜, 等. 海洋枝顶孢霉BH0531发酵液对根结线虫卵及二龄幼虫的影响[J]. 中国酿造, 2017, 36(4): 26-29. doi: 10.11882/j.issn.0254-5071.2017.04.006
[34]	赵迪. 菌株Snef5杀线虫活性物质分离纯化及相关活性基因簇分析[D]. 沈阳: 沈阳农业大学, 2013.
[35]	李玲玉, 赵迪, 刘晓宇, 等. 放线菌Snea253代谢产物及寡糖对南方根结线虫活性的影响[J]. 天然产物研究与开发, 2014, 26(1): 117-122.
[36]	NIU Q, HUANG X, ZHANG L, et al. A neutral protease from Bacillus nematocida, another potential virulence factor in the infection against nematodes[J]. Arch Microbiol, 2006, 185(6): 439-448. doi: 10.1007/s00203-006-0112-x
[37]	刘霆, 王莉, 段玉玺, 等. Snf907真菌代谢物对大豆胞囊线虫卵及二龄幼虫的影响[J]. 大豆科学, 2006, 25(3): 325-328. doi: 10.3969/j.issn.1000-9841.2006.03.024
[38]	许艳丽, 鲁建聪, 潘凤娟. 混合寄生真菌发酵液对大豆胞囊线虫的毒力和防效[J]. 土壤与作物, 2018, 7(2): 248-256. doi: 10.11689/j.issn.2095－2961.2018.02.019

Cited By

Cited by

Periodical cited type(3)

1.	马逸平，房志琴，李彩榕，王亚玲. 内科支架复合材料的制备与老化性能. 金属功能材料. 2023(04): 105-112 .
2.	杨继全，薛政龙，李娜，施建平，唐文来，张钢. 基于3D打印的电工电子材料. 机械设计与制造工程. 2022(04): 1-6 .
3.	李超凡，白海清，杨思瑞，任礼，贾仕奎. 聚乳酸3D打印螺杆设计及挤出仿真分析. 工程塑料应用. 2022(05): 94-100 .

Other cited types(4)

Get Citation

PDF

XML

Article views (887) PDF downloads (1124) Cited by(7)

Turn off MathJax

Article Contents

Abstract

References

Identification of two fungi strains and their control effects to cereal cyst nematode

Abstract

References

Cited by

Periodical cited type(3)

Other cited types(4)

Catalog

Export File

Citation

Format

Content