Effect of <i>pagP</i> gene deletion on outer membrane characteristics of avian pathogenic <i>Escherichia coli</i>

SHU Lingling; XU Liuliu; QI Kezong; TU Jian; SONG Xiangjun; SHAO Ying

doi:10.7671/j.issn.1001-411X.201811038

Journal of South China Agricultural University > 2019 > 40(6): 8-14. > DOI: 10.7671/j.issn.1001-411X.201811038

SHU Lingling, XU Liuliu, QI Kezong, et al. Effect of pagP gene deletion on outer membrane characteristics of avian pathogenic Escherichia coli[J]. Journal of South China Agricultural University, 2019, 40(6): 8-14. DOI: 10.7671/j.issn.1001-411X.201811038

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Effect of pagP gene deletion on outer membrane characteristics of avian pathogenic Escherichia coli

College of Animal Science and Technology, Anhui Agricultural University/Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Hefei 230036, China

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Received Date: November 24, 2018
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To study the effect of pagP gene deletion on outer membrane characteristics of avian pathogenic Escherichia coli(APEC).

Method
The minimal inhibitory concentration (MIC) experiment was used to explore the effect of pagP deletion on the permeability of outer membrane of strain. Autoaggregation experiment, outer membrane hydrophobicity test, and analysis of biofilm-forming conditions of strain were performed to understand the influence of pagP deletion on biofilm-forming ability. The biofilm morphology was observed by scanning electron microscopy.

Result
The MIC of strain decreased after the deletion of pagP gene, the outer membrane permeability of strain increased, and the autoaggregation ability of strain increased significantly (P<0.01). The MICs of erythromycin and ampicillin were 7 and 20 μg/mL respectively. The autoaggregation ability of strain was 87.89%. The deletion ofpagP gene had no significant effect on the hydrophobicity of outer membrane of strain, and the hydrophobicity was only 5.337%. The amount of biofilm increased with the increase of time when the strain was cultured in LB medium. The strain with pagP deletion had higher biofilm-forming ability than wild strain.

Conclusion
The deletion of pagP gene can change the characteristics of outer membrane of APEC and enhance the biofilm-forming ability.
- avian pathogenic Escherichia coli,
- pagP gene,
- biofilm,
- hydrophobicity,
- autoaggregation ability

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References (32)

References

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Effect of pagP gene deletion on outer membrane characteristics of avian pathogenic Escherichia coli

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