QTL analysis of low nitrogen tolerance in rice seedlings based on chromosome segment substitution lines

JIA Peilong; LI Biao; LI Minghui; LIU Jianbin; LI Rongbai; LUO Jijing

doi:10.7671/j.issn.1001-411X.201810046

Journal of South China Agricultural University > 2019 > 40(4): 16-24. > DOI: 10.7671/j.issn.1001-411X.201810046

JIA Peilong, LI Biao, LI Minghui, et al. QTL analysis of low nitrogen tolerance in rice seedlings based on chromosome segment substitution lines[J]. Journal of South China Agricultural University, 2019, 40(4): 16-24. DOI: 10.7671/j.issn.1001-411X.201810046

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QTL analysis of low nitrogen tolerance in rice seedlings based on chromosome segment substitution lines

JIA Peilong^1,,
LI Biao²,
LI Minghui²,
LIU Jianbin²,
LI Rongbai^{1, 3, ,},
LUO Jijing^{2, 3, ,}

1.
College of Agriculture, Guangxi University, Nanning 530004, China
2.
College of Life Science and Technology, Guangxi University, Nanning 530004, China
3.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China

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Received Date: October 27, 2018
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To map the quantitative trait loci (QTL) associated with low nitrogen tolerance in rice (Oryza sativa), provide a basis for future fine mapping, cloning and functional characterization of related genes and offer theoretical references for breeding of low nitrogen tolerant rice.

Method
A set of chromosomal segment substitution lines which was constructed by crossing Koshihikari (recurrent parent) and Nona Bokra (donor parent) were used as test materials. Low nitrogen stress treatment was applied at rice seedling stage. The relative loss ratios of eight phenotypes including rice plant height, root length, root fresh weight, root dry weight, shoot fresh weight, shoot dry weight, total fresh weight and total dry weight were analyzed, and QTL mapping was performed.

Result
Two loci related to low nitrogen tolerance of rice were successfully mapped. These two QTLs were qRL1-1 and qRFW2-1 which were related to root length and root fresh weight respectively under low nitrogen stress. qRL1-1 was closed to the M1-29 marker on chromosome 1 with a LOD score of 2.89 and explained about 11.23% of total phenotypic variance. qRFW2-1 was closed to the M2-225 marker on chromosome 2 with a LOD score of 2.53 and explained about 9.90% of total phenotypic variance. No loci was found for other six phenotypic indexes.

Conclusion
QTLs related to root length and root fresh weight under low nitrogen stress are mapped, which lays the foundation for further genetic fine mapping and cloning of the underlying genes.
- Oryza sativa,
- chromosomal segment substitution line,
- seedling stage,
- low nitrogen tolerance,
- QTL mapping

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