Molecular detection of PRRSV and variation analysis of <i>ORF5</i> gene in Fujian province in 2017

WEI Yingfang; JI Chihai; SUN Yankuo; HAN Xiaoliang; CHEN Yao; ZHANG Guihong

doi:10.7671/j.issn.1001-411X.201810027

Journal of South China Agricultural University > 2019 > 40(3): 14-19. > DOI: 10.7671/j.issn.1001-411X.201810027

WEI Yingfang, JI Chihai, SUN Yankuo, et al. Molecular detection of PRRSV and variation analysis of ORF5 gene in Fujian province in 2017[J]. Journal of South China Agricultural University, 2019, 40(3): 14-19. DOI: 10.7671/j.issn.1001-411X.201810027

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Molecular detection of PRRSV and variation analysis of ORF5 gene in Fujian province in 2017

1.
National Engineering Research Center for Breeing Swine Industry/Key Laboratory of Zoonosis Prevention and Control of Guangdong Province/College of Veterinary, South China Agricultural University, Guangzhou 510642, China
2.
Jiangsu Hengfengqiang Biotechnology Co., Ltd., Nantong 226100, China

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Received Date: October 17, 2018
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To study the molecular prevalence of the porcine peproductive and respiratory syndrome virus (PRRSV) strain in small and medium-sized pig farms of Fujian province in 2017.

Method
We collected 83 suspected blue ear positive disease materials from small and medium-sized pig farms in Fujian province. RT-PCR detection and genetic evolution analysis of ORF5 gene which encoded GP5 protein was performed.

Result
The positive rate of 83 suspected blue ear disease samples was 73%, and the nucleotide similarity among the measured ORF5sequences was 80.3%−100.0%. The nucleotide similarities between tested strains of different branches and the representative strains of those branches, NADC30, GM2 and JXA1, were 92.6%−93.9%, 89.8%−93.0% and 93.4%−99.3%, respectively. The new identified strain in the new branch had nucleotide similarity of 91.0%−93.0% to JXA1. The GP5 amino acid alignment showed significant variation in functional regions such as GP5 signal peptide region, induction epitope, principal neutralizing epitope and hypervariable region in different subgroups.

Conclusion
A new branch of PRRSV has emerged in Fujian area, making the PRRSV variants in Fujian more diverse. It is necessary to strengthen the monitoring of PRRSV epidemics and mutations.
- porcine reproductive and respiratory syndrome,
- blue ear disease,
- molecular epidemiology,
- ORF5gene,
- genetic variation

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References (19)

References

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Molecular detection of PRRSV and variation analysis of ORF5 gene in Fujian province in 2017