Secondary metabolites of endophytic fungi isolated from <i>Casuarina equisetifolia</i> and their bioactivities

SHAN Tijiang; QIN Kai; XIE Yinyan; ZHANG Weihao; MAO Ziling; WANG Jun

doi:10.7671/j.issn.1001-411X.201807040

Journal of South China Agricultural University > 2019 > 40(3): 67-74. > DOI: 10.7671/j.issn.1001-411X.201807040

SHAN Tijiang, QIN Kai, XIE Yinyan, et al. Secondary metabolites of endophytic fungi isolated from Casuarina equisetifolia and their bioactivities[J]. Journal of South China Agricultural University, 2019, 40(3): 67-74. DOI: 10.7671/j.issn.1001-411X.201807040

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Secondary metabolites of endophytic fungi isolated from Casuarina equisetifolia and their bioactivities

College of Forestry and Landscape Architecture/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China

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Received Date: July 23, 2018
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To isolate and identify endophytic fungi from branches and fruits of Casuarina equisetifolia Forst., and screen active endophytic fungi with antibacterial and antioxidant activities.

Method
Endophytic fungi from branches and fruits of C. equisetifolia were isolated by the tissue block separation method, and identified by combining colony observation with molecular biology. The antibacterial activities of secondary metabolites produced by endophytic fungi against different bacteria were determined using TLC-bioautography and their antioxidant activities were determined using the multi-orifice plate-DPPH coloration method.

Result
Twelve strains were isolated and identified from branches and fruits of C. equisetifolia. There were significant difference between endophytic fungi isolated from branches and fruits. There were only two same endophytic fungus strains in branches and fruits, namely Cef-1 (Pseudofusicoccum sp.) and Cef-7 (Pestalotiopsis sp.). All endophytic fungi were mainly distributed in nine different genera, including Pseudofusicoccum, Botryosphaeria, Pestalotiopsis, Trichoderma, Aspergillus, Rhytidhysteron, Phomopsis, Fusarium and Colletotrichum. Among them, Botryosphaeria had the most species and was the dominant species, followed by Pestalotiopsis. Cef-2 (Botryosphaeria sp.) showed the strongest inhibitory activity and the diameters of antibacterial spots were more than 10 mm, which were stronger than that of the positive control streptomycin sulfate. Cef-1 and Cef-9 (Fusarium sp.) showed well inhibition on Ralstonia solanacearum. Cef-2 displayed the best antioxidant activity and the IC₅₀ value was 0.80 mg/mL.

Conclusion
There are endophytic fungi in branches and fruits of Casuarina equisetifolia, and Botryosphaeria is the dominant species. There are significant differences of fungi species between branches and fruits. Endophytic fungus Cef-2 exhibits the strongest antibacterial and antioxidant activities, which can be used as a candidate strain for further isolation and identification of active component.
- Casuarina equisetifolia,
- endophytic fungus,
- secondary metabolite,
- antibacterial activity,
- antioxidant activity

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References (33)

References

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