Genetic analysis and mapping of bacterial leaf streak resistance genes in <i>Oryzae rufipogon</i> Griff

SHI Lijun; LUO Dengjie; ZHAO Yan; CEN Zhenlu; LIU Fang; LI Rongbai

doi:10.7671/j.issn.1001-411X.201805009

Journal of South China Agricultural University > 2019 > 40(2): 1-5. > DOI: 10.7671/j.issn.1001-411X.201805009

SHI Lijun, LUO Dengjie, ZHAO Yan, et al. Genetic analysis and mapping of bacterial leaf streak resistance genes in Oryzae rufipogon Griff[J]. Journal of South China Agricultural University, 2019, 40(2): 1-5. DOI: 10.7671/j.issn.1001-411X.201805009

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Genetic analysis and mapping of bacterial leaf streak resistance genes in Oryzae rufipogon Griff

SHI Lijun^{1, 2,},
LUO Dengjie¹,
ZHAO Yan¹,
CEN Zhenlu³,
LIU Fang^1, ,,
LI Rongbai^1, ,

1.
College of Agriculture, Guangxi University/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530005, China
2.
Guangxi Medical Botanical Garden, Nanning 530023, China
3.
Microorganism Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

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Received Date: May 15, 2018
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To exploit major resistance genes of rice (Oryza sativa L.) to bacterial leaf streak (BLS), and provide a basis for enriching rice disease resistant gene resources and breeding resistant cultivars.

Method
Constructing a mapping population, we conducted genetic analyses of BLS resistance genes in Oryzae rufipogon Griff ‘DY19’ using the bulk segregant analysis method, and preliminarily mapped the BLS resistance gene bls2 through SSR molecular markers.

Result
The ratio of resistant and susceptible individual plants in F₂ population was in accordance with the theoretical segregation ratio of 1∶3, which showed that the resistance gene to BLS in O. rufipogon ‘DY19’ was consistent with single gene genetic pattern and controlled by one pair of main recessive gene bls2. This gene was mapped in a 4 cM region between molecular markers SL03 (23 474 851 bp) and SL04 (24 484 154 bp) on the second chromosome. This locus was a new disease resistance locus.

Conclusion
The BLS resistance in O. rufipogon ‘DY19’ is controlled by one pair of new major recessive gene bls2. It can provide a reference for further fine mapping.
- rice,
- bacterial leaf streak,
- resistant gene,
- genetic analysis,
- gene mapping

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References

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