Increase of 10-deacetyl baccatin Ⅲ-10<i>β</i>-O-acetyl transferase activity with molecular docking simulation and site-directed mutation

OUYANG Pinglan; HUANG Jiajun; LIN Shuling; WEI Tao; LIN Junfang; GUO Liqiong; LIU Junfeng; LIU Qiqian

doi:10.7671/j.issn.1001-411X.2018.05.013

Journal of South China Agricultural University > 2018 > 39(5): 87-92. > DOI: 10.7671/j.issn.1001-411X.2018.05.013

OUYANG Pinglan, HUANG Jiajun, LIN Shuling, WEI Tao, LIN Junfang, GUO Liqiong, LIU Junfeng, LIU Qiqian. Increase of 10-deacetyl baccatin Ⅲ-10β-O-acetyl transferase activity with molecular docking simulation and site-directed mutation[J]. Journal of South China Agricultural University, 2018, 39(5): 87-92. DOI: 10.7671/j.issn.1001-411X.2018.05.013

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Increase of 10-deacetyl baccatin Ⅲ-10β-O-acetyl transferase activity with molecular docking simulation and site-directed mutation

OUYANG Pinglan^{1, 2,},
HUANG Jiajun^{1, 2},
LIN Shuling^{1, 2},
WEI Tao^{1, 2},
LIN Junfang^{1, 2, ,},
GUO Liqiong^{1, 2, ,},
LIU Junfeng¹,
LIU Qiqian¹

1.
College of Food Science, South China Agricultural University, Guangzhou 510642, China
2.
Research Center for Microecologics Engineering of Guangdong Province, Guangzhou 510640, China

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Received Date: January 28, 2018
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To increase the activity of 10-deacetyl baccatin Ⅲ-10β-O-acetyl transferase (DBAT) and the efficiency of in vitro enzymatic synthesis of baccatin Ⅲ.

Method
Computer simulation of molecular docking between DBAT and its natural substrate acetyl CoA was performed. Three amino acid residues including C165W, N300I and F160C were chosen for site-directed mutation. The recombinant mutants were expressed in Escherichia coli and their enzymatic properties were determined by reactions in vitro.

Result
The relative molecular mass of each of the three mutant enzymes was 67 000, and the optimum reaction temperature was 32.5 ℃ which was consistant with that of the wild type enzyme (DBAT). The optimum reaction pH of DBAT^F160C or DBAT was 7.5, slightly higher than that of DBAT^C165W or DBAT^N300I (pH 7.0). Compared with DBAT, DBAT^C165W, DBAT^F160C and DBAT^N300I had 61.5%, 59.6% and 19.2% higher specific activities, 55.4%、35.1% and 2.9% higher catalytic efficiency, as well as higher K_m and v_max.

Conclusion
Hydrophobic mutations lead to significantly increased specific activities for mutant enzymes compared with the wild type enzyme. This study provides a basis for efficient in vitro enzymatic synthesis of baccatin Ⅲ, a direct precursor for chemical semi-synthesis of anti-cancer drug paclitaxel.
- 10-deacetyl baccatin III-10β-O-acetyl transferase,
- molecular docking,
- site-directed mutation,
- specific activity,
- enzymatic reaction,
- baccatin Ⅲ

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References

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Increase of 10-deacetyl baccatin Ⅲ-10β-O-acetyl transferase activity with molecular docking simulation and site-directed mutation

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