Inhibitory effects of Bacillus amyloliquefaciens gfj-4 fermentation supernatant and its mixtures with chemical fungicides against Exserohilum turcicum

    More Information
    • Received Date: March 25, 2018
    • Available Online: May 17, 2023
    • Objective 

      To elucidate the inhibitory activities of the secondary metabolites of Bacillus amyloliquefaciens gfj-4 and their mixtures with chemical fungicides against Exserohilum turcicum.

      Method 

      Based on the mycelium growth rate, we determined the effects of different culture and fermentation times of seed broth and fermentation time on the production of antifungal substances, and evaluated the inhibition effects of fermentation supernatant, lipopeptide crude extracts, and mixtures of fermentation supernatant and seven chemical fungicides.

      Result 

      The most suitable incubation time of seed broth was 6 h and the appropriate fermentation time was 72 h. The EC50 against E. turcicum of the fermentation supernatant and lipopeptide crude extract were 0.32 and 0.11 μL·mL–1, respectively. E. turcicum showed a high sensitivity to difenoconazole, tebuconazole, procymidone and myclobutanil. Among them, difenoconazole had the highest inhibitory activity against E. turcicum with EC50 of 0.10 μg·mL–1. Themancozeb and thiram had similar inhibitory activities with EC50 of 12.03 and 12.08 μg·mL–1, respectively. The inhibitory effect of propineb on E. turcicum was the worst with EC50 of 24.73 μg·mL–1. The gfj-4 fermentation supernatant and difenoconazole had the additive effect, and the mixture with a volume ratio of 3∶7 had the highest toxicity ratio of 1.28. The fermentation supernatant mixed with mancozeb or propineb in a volume ratio of 2∶8 both had the highest toxicity ratio of 1.28 or 1.67, respectively.

      Conclusion 

      B. amyloliquefaciens gfj-4 and its mixture with difenoconazole or propineb have important application values in pesticide reduction and synergism.

    • [1]
      冯胜泽, 刘星晨, 王海祥, 等. 玉米大斑病菌分生孢子形成的影响因素及GATA转录因子家族的表达分析[J]. 中国农业科学, 2017, 50(7): 1234-1241.
      [2]
      王晓鸣, 巩双印, 柳家友, 等. 玉米叶斑病药剂防控技术探索[J]. 作物杂志, 2015(3): 150-154.
      [3]
      陈乐乐, 郭贝贝, 李北兴, 等. 四霉素对番茄叶霉病菌的毒力效应及田间防治效果[J]. 农药学学报, 2017, 19(3): 324-330.
      [4]
      雷仲仁. 病虫害生物防治是实现蔬菜安全生产的主要途径[J]. 中国农业科学, 2016, 49(15): 2932-2934.
      [5]
      张国军. 八种常用杀菌剂“三致”作用及生殖毒性研究进展[J]. 中国预防医学杂志, 2007, 8(3): 320-321.
      [6]
      王宣, 黄涛珍. 农药污染问题及对策研究[J]. 陕西农业科学, 2016, 62(10): 108-111.
      [7]
      严婉荣, 赵廷昌, 肖彤斌, 等. 生防细菌在植物病害防治中的应用[J]. 基因组学与应用生物学, 2013, 32(4): 533-539.
      [8]
      张红娟, 卢海波, 赵丽娟, 等. 生防菌HT3和HT5代谢产物对灰葡萄孢菌的抑菌作用[J]. 山西农业科学, 2013, 41(12): 1372-1375.
      [9]
      楚文琢, 彭双强, 廖晓兰, 等. 铜绿假单胞菌SU8发酵液与乙蒜素混配对草莓灰霉病的防效[J]. 江苏农业科学, 2017, 45(6): 79-83.
      [10]
      孙东磊, 管楚雄, 曾杨, 等. 发光杆菌1029发酵液和乙膦铝混配对荔枝霜疫霉病菌的抑制作用[J]. 广东农业科学, 2009, 36(9): 105-107.
      [11]
      张龙来, 康向辉, 魏孝义, 等. 1株解淀粉芽孢杆菌HN011 抑菌次级代谢产物的分析[J]. 华南农业大学学报, 2016, 37(1): 63-69.
      [12]
      中华人民共和国农业部. 农药室内生物测定试验准则(杀菌剂)第2部分: 抑制病原真菌菌丝生长试验平皿法: NY/T 1156.2—2006[S]. 北京: 农业部市场与经济信息司, 2006: 1-6.
      [13]
      邓建良, 刘红彦, 刘玉霞, 等. 解淀粉芽孢杆菌YN-1抑制植物病原真菌活性物质鉴定[J]. 植物病理学报, 2010, 40(2): 202-209.
      [14]
      陈福良, 郑斐能, 王仪. 农药混配室内毒力测定的一种实验技术[J]. 农药科学与管理, 1997(4): 30-34.
      [15]
      秦虎强, 陈芳颖, 付鼎程, 等. 油菜菌核病菌对10种杀菌剂的敏感性及不同药剂田间防效[J]. 西北农林科技大学学报(自然科学版), 2011, 39(7): 117-122.
      [16]
      郭建国, 杨凤珍, 杜蕙, 等. 甘肃玉米大斑病菌对嘧菌酯的敏感基线与抗药性监测[J]. 植物保护学报, 2015, 42(6): 1044-1049.
      [17]
      刘杰, 姜玉英, 曾娟. 2012年玉米大斑病重发原因和控制对策[J]. 植物保护, 2013, 39(6): 86-90.
      [18]
      赵淑莉, 任飞娥, 刘金亮, 等. 玉米大斑病生防放线菌的筛选鉴定及发酵条件优化[J]. 微生物学报, 2012, 52(10): 1228-1236.
      [19]
      沈玲, 朱欣洁, 王恒超, 等. 放线菌菌株AH-1的分离鉴定与抑菌活性研究[J]. 西北农业学报, 2015, 24(5): 128-132.
      [20]
      蒋细良, 谢德龄. 农用抗生素的作用机理[J]. 生物防治通报, 1994, 10(2): 76-81.
      [21]
      孙延忠, 曾洪梅, 石义萍, 等. 武夷菌素对番茄灰霉菌(Botrytis cinerea)的作用方式[J]. 植物病理学报, 2003, 33(5): 434-438.
      [22]
      牛慧芹, 刘春辉, 沈检龙, 等. 玉米大斑病生防细菌的筛选、鉴定及其抑制作用[J]. 中国农学通报, 2014, 30(28): 275-279.
      [23]
      候美玲, 辛媛媛, 郝志敏, 等. 玉米内生芽胞杆菌的抗菌活性物质及其拮抗玉米大斑病菌机理的初步研究[J]. 农业生物技术学报, 2012, 20(9): 1018-1027.
      [24]
      ONGENA M, JACQUES P. Bacillus lipopeptides: Versatile weapons for plant disease biocontrol[J]. Trends Microbiol, 2008, 16(3): 115-125.
      [25]
      陈中义, 张杰, 黄大昉. 植物病害生防芽孢杆菌抗菌机制与遗传改良研究[J]. 植物病理学报, 2003, 33(2): 97-103.
      [26]
      侯红漫, 靳艳, 金美芳, 等. 环脂肽类生物表面活性剂结构、功能及生物合成[J]. 微生物学通报, 2006, 33(5): 122-128.
      [27]
      ARREBOLA E, JACOBS R, KORSTEN L. Iturin A is the principal inhibitor in the biocontrol activity of Bacillus amyloliquefaciens PPCB004 against postharvest fungal pathogens[J]. J Appl Microbiol, 2010, 108(2): 386-395.
      [28]
      李宝庆, 鹿秀云, 郭庆港, 等. 枯草芽孢杆菌BAB-1产脂肽类及挥发性物质的分离和鉴定[J]. 中国农业科学, 2010, 43(17): 3547-3554.
      [29]
      王帅. 芽孢杆菌及其脂肽类化合物防治植物病害和促进植物生长的研究[D]. 南京: 南京农业大学, 2009.
      [30]
      刘邮洲, 陈夕军, 尹小乐, 等. 23株芽胞杆菌及其脂肽类化合物抑菌活性比较[J]. 江苏农业学报, 2017, 33(3): 533-542.
      [31]
      王文桥, 马志强, 张小风, 等. 植物病原菌对杀菌剂抗性风险评估[J]. 农药学学报, 2001, 3(1): 6-11.
      [32]
      王国平, 曾邵平, 徐彬, 等. 木霉菌素和嘧霉胺复配剂防治抗药性灰霉病[J]. 农药, 2013, 52(5): 377-379.
      [33]
      王中华, 杨青松, 蔺经, 等. 鲍曼菌素对梨黑斑病菌的毒力及药效评价[J]. 南方农业学报, 2011, 42(10): 1217-1220.

    Catalog

      Article views (1748) PDF downloads (2255) Cited by()

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return