- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
- Journal of Citation Report of Chinese S&T Journals (Core Edition)
| Citation: |
ZHOU Pengfei, ZHANG Jing, ZHANG Bowen, HU Qiongbo. Genetic geographical difference of Isaria fumosorosea and its pathogenicity against B-biotype Bemisia tabaci[J]. Journal of South China Agricultural University, 2018, 39(3): 60-64. DOI: 10.7671/j.issn.1001-411X.2018.03.010
|
To study the genetic geographical difference of Isaria fumosorosea strains isolated from different regions, and the pathogenicity of I. fumosorosea to B-biotype Bemisia tabaci.
Sixteen strains of I. fumosorosea were isolated from soils of Guangdong, Fujian, and Qinghai. The bioinformatics analysis was used to identify ITS sequences similarities of the 16 strains, and the pathogenetic tree was constructed. The immersion method was employed to evaluate the pathogenicities of I. fumosorosea strains against second-instar nymphs of B-biotype B. tabaci.
The ITS sequences similarities between the test I. fumosorosea strains and the reference strains(KX057373.1, KX057375.1) were over 97.7%. Nine strains from Guangdong and six strains from Fujian had no geographic difference. The ITS sequence of Qinghai strain IfTS01 was almost the same as that of reference strain (KX057375.1), and highly similar to the strain IfFJ02、IfFJ06 of Fujian and strain IfGD17 of Guangdong. The fatality rate of B-biotype B. tabaci ranged from 47% to 86% after 10 days treatment with 10 mL–1 conidia of the 16 I. fumosorosea strains. The most effective strains, IfFJ06, IfGD02 and IfTS01, caused above 80% fatality rate of B. tabaci.
There is no obvious difference of genetic geography among ITS sequences of I. fumosorosea strains isolated from different areas. The pathogenicity of I. fumosorosea strains aganist B. tabaci is not related to its geographical distribution and ITS sequence similarity. There are three strains with high pathogenicity to B. tabaci, which can be used for further research.
| [1] |
BROWN J K, FROHLICH D R, ROSELL R C. The sweetpotato or silverleaf whiteflies: Biotypes of Bemisia tabaci or a species complex?[J]. Annu Rev Entomol, 2003, 40(1): 511-534.
|
| [2] |
BROWN J K, CZOSNEK H. Whitefly transmission of plant viruses[J]. Adv Bot Res, 2002, 36(2): 65-76.
|
| [3] |
李庆亮, 谭伟, 薛明. B型烟粉虱危害对烟草叶片光系统Ⅱ的影响[J]. 中国农业科学, 2012, 45(19): 3988-3995.
|
| [4] |
张友军, 朱国仁, 褚栋, 等. 我国蔬菜作物重大入侵害虫发生、危害与控制[J]. 植物保护, 2011, 37(4): 1-6.
|
| [5] |
DONG T Y, ZHANG B W, JIANG Y F, et al. Isolation and classification of fungal whitefly entomopathogens from soils of Qinghai-Tibet Plateau and Gansu Corridor in China[J]. Plos One, 2016, 11(5): e0156087.
|
| [6] |
ZHANG B W, ZOU C, HU Q B. Effects of Isaria fumosorosea on TYLCV (Tomato yellow leaf curl virus) accumulation and transmitting capacity of Bemisia tabaci[J]. PloS One, 2016, 11(10): e0164356.
|
| [7] |
HU Q B, WU W. Recombinant fungal entomopathogen RNAi target insect gene[J]. Bioengineered, 2016, 7(6): 504-507.
|
| [8] |
吴丹丹, 黄少华, 章玉苹, 等. 玫烟色拟青霉固体培养基筛选及培养条件研究[J]. 广东农业科学, 2015, 42(15): 43-47.
|
| [9] |
吕利华, 武亚敬, 何余容, 等. 玫烟色拟青霉亚致死浓度对小菜蛾取食和成虫生殖的影响[J]. 中国生物防治学报, 2007, 23(4): 322-327.
|
| [10] |
WRAIGHT S P, CARRUTHERS R I, JARONSKI S T, et al. Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly, Bemisia argentifolii[J]. Biol Control, 2000, 17(3): 203-217.
|
| [11] |
SHI W B, FENG M G. Lethal effect of Beauveria bassiana, Metarhizium anisopliae, and Paecilomyces fumosoroseus on the eggs of Tetranychus cinnabarinus (Acari: Tetranychidae) with a description of a mite egg bioassay system[J]. Biol Control, 2004, 30(2): 165-173.
|
| [12] |
HU Q B, LIU S Y, YIN F, et al. Diversity and virulence of soil-dwelling fungi Isaria spp. and Paecilomyces spp. against Solenopsis invicta (Hymenoptera: Formicidae)[J]. Biocontrol Sci Techn, 2011, 21(2): 225-234.
|
| [13] |
ZHOU F, SHAUKAT A, HUANG Z. Influence of the entomopathogenic fungus Isaria fumosorosea on Axinoscymnus cardilobus (Coleoptera: Coccinellidae) under laboratory conditions[J]. Biocontrol Sci Techn, 2010, 20(7): 709-722.
|
| [14] |
梅利, 朱振宇, 刘永翔, 等. 玫烟色棒束孢几丁质酶基因部分序列的克隆及分析[J]. 贵州农业科学, 2010, 38(3): 19-22.
|
| [15] |
ABBOTT W S. A method of computing the effectiveness of an insecticide[J]. J Econ Entomol, 1925, 18(2): 265-267.
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: