- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
- Journal of Citation Report of Chinese S&T Journals (Core Edition)
| Citation: |
YANG Linlin, ZHANG Tao, YANG Limin, HAN Mei. Correlation analyses of ecological factors, ginsenoside contents and expression of associated key enzymes in Panax ginseng leaves[J]. Journal of South China Agricultural University, 2018, 39(3): 39-47. DOI: 10.7671/j.issn.1001-411X.2018.03.007
|
To clarify the physiological and ecological mechanisms of ginsenoside biosynthesis, and reveal the relationship between ecdogical/genetic factors and quality of medicinal materials.
Cultivated four-year-old Panax ginseng leaves at different growth times were used as materials. The contents of eight ginsenosides (Rg1、Re、Rf、Rb1、Rb2、Rb3、Rc、Rd) in leaves were detected by HPLC. The expression of seven key enzyme genes (HMGR, FPS, SS, SE, DS, β-AS, CYP716A47) involved in ginsenoside biosynthesis in ginseng leaves were determined by real-time quantitative fluorescent PCR. The effect of ecological factors and the expression of key enzyme genes on the synthesis and accumulation of ginsenosides in P. ginseng leaves were investigated by correlation and grey relational analysis.
The expression of key enzyme genes involved in ginsenoside synthesis was active from July 13th to September 29th, and the expression of different genes synergistically increased or decreased. The contents of ginsenoside Re and Rg1 in P. ginseng leaves were the highest, reached the maximum of 60.30 and 39.38 mg·g–1 respectively in the growth period of ginseng root from August 31st to September 13th. Temperature, photosynthetic active radiation (PAR), soil water potential and relative humidity all had significant correlation with ginsenoside contents in P. ginseng leaves (P<0.05).HMGR expression in P. ginseng leaves was negatively correlated with Rb2 content (P<0.05),SS expression was negatively correlated with Rg1 or Re content (P<0.05), andβ-AS expression was negatively correlated with Rc content (P<0.05) and Rb2 content (P<0.01). Grey correlation analysis results showed that the major ecological factors that influenced ginsenoside contents inP. ginseng leaves were temperature, PAR, soil water potential and relative humidity, with their grey correlation values ranging from 0.727 9 to 0.871 1.
Under the regulation and control of ecological factors, the synthesis and accumulation of ginsenosides were influenced by the expression of involved key enzyme genes.
| [1] |
黎阳, 张铁军, 刘素香, 等. 人参化学成分和药理研究进展[J]. 中草药, 2009, 40(1): 164-166.
|
| [2] |
逄世峰, 李亚丽, 许世泉, 等. 人参不同部位人参皂苷类成分研究[J]. 人参研究, 2015, 27(1): 5-8.
|
| [3] |
张兰兰, 高文远, 宋兆辉, 等. 不同年份林下山参叶中皂苷类成分含量变化研究[J]. 中国中药杂志, 2012, 37(17): 2530-2533.
|
| [4] |
ZHAO C L, CUI X M, CHEN Y P, et al. Key enzymes of triterpenoid saponin biosynthesis and the induction of their activities and gene expressions in plants[J]. Nat Prod Commun, 2010, 5(7): 1147-1158.
|
| [5] |
HAN J Y, KIM H J, KWON Y S, et al. The Cyt P450 enzyme CYP716A47 catalyzes the formation of protopanaxadiol from dammarenediol-II during ginsenoside biosynthesis in Panax ginseng[J]. Plant Cell Physiol, 2011, 52(12): 2062-2073.
|
| [6] |
SAWAI S, SAITO K. Triterpenoid biosynthesis and engineering in plants[J]. Front Plant Sci, 2011. doi: 10.3389/fpls.2011.00025
|
| [7] |
林彦萍, 张美萍, 王康宇, 等. 人参皂苷生物合成研究进展[J]. 中国中药杂志, 2016, 41(23): 4292-4302.
|
| [8] |
杨利民. 中药资源生态学及其科学问题[J]. 吉林农业大学学报, 2008, 30(4): 506-510.
|
| [9] |
焦晓林, 高微微. 环境因子对药用植物三萜皂苷合成影响的研究进展[J]. 中草药, 2011, 42(2): 398-402.
|
| [10] |
LIU J, WANG Q, SUN M, et al. Selection of reference genes for quantitative real-time PCR normalization in Panax ginseng at different stages of growth and in different organs[J]. PLoS One, 2014, 9(11): e112177.
|
| [11] |
杨怡姝, 孙晓娜, 王小利, 等. 实时荧光定量PCR技术的操作实践[J]. 实验室研究与探索, 2011, 30(7): 15-19.
|
| [12] |
林红梅, 张永刚, 李岳桦, 等. 不同产地人参中8种人参单体皂苷含量比较[J]. 特产研究, 2013, 35(4): 63-66.
|
| [13] |
杨利民, 张永刚, 林红梅, 等. 中药材质量形成理论与控制技术研究进展[J]. 吉林农业大学学报, 2012, 34(2): 119-124.
|
| [14] |
谢彩香, 索风梅, 贾光林, 等. 人参皂苷与生态因子的相关性[J]. 生态学报, 2011, 31(24): 7551-7563.
|
| [15] |
贾光林, 黄林芳, 索风梅, 等. 人参药材中人参皂苷与生态因子的相关性及人参生态区划[J]. 植物生态学报, 2012, 36(4): 302-312.
|
| [16] |
张治安, 徐克章, 任跃英, 等. 光照条件对参株碳水化合物和人参皂甙含量的影响[J]. 吉林农业大学学报, 1994, 16(3): 15-17.
|
| [17] |
王红星, 乔传英, 古红梅. 影响植物次生代谢产物形成的因素[J]. 安徽农业科学, 2007, 35(35): 11376-11377.
|
| [18] |
包京姗, 王秀全, 金晶茹, 等. 不同土壤及其含水量对人参生长的影响[J]. 吉林农业大学学报, 2009, 31(6): 725-728.
|
| [19] |
YU Q, WANG T. Simulation of the physiological responses of C3 plant leaves to environmental factors by a model which combines stomatal conductance, photosynthesis and transpiration[J]. Acta Bot Sin, 1998, 40(8): 740-750.
|
| [20] |
KIM Y J, LEE O R, OH J Y, et al. Functional analysis of 3-hydroxy-3-methylglutaryl coenzyme a reductase encoding genes in triterpene saponin-producing ginseng[J]. Plant Physiol, 2014, 165(1): 373-387.
|
| [21] |
PARK H W, KIM O T, HYUN D W, et al. Overexpression of farnesyl diphosphate synthase by introducing CaFPS gene in Panax ginseng C. A. Mey[J]. Korea Journal of Medicinal Crop Science, 2013, 21(1): 32-38.
|
| [22] |
蒋世翠, 张明哲, 王义, 等. 人参SQS基因的干扰载体构建及转化人参愈伤组织[J]. 吉林大学学报(理学版), 2011, 49(6): 1136-1140.
|
| [23] |
HAN J Y, IN J G, KWON Y S, et al. Regulation of ginsenoside and phytosterol biosynthesis by RNA interferences of squalene epoxidase gene in Panax ginseng[J]. Phytochemistry, 2010, 71(1): 36-46.
|
| [24] |
HAN J Y, KWON Y S, YANG D C, et al. Expression and RNA interference-induced silencing of the dammarenediol synthase gene in Panax ginseng[J]. Plant Cell Physiol, 2006, 47(12): 1653-1662.
|
| [25] |
刘娟, 纪瑞锋, 陈同, 等. 人参皂苷生物合成基因组织表达特性的研究[J]. 中国中药杂志, 2017, 42(13): 2453-2459.
|
| [1] | CHEN Xiang, LIU Yiyang, LUO Lu, CHENG Guoxin, GUO Meng, GAO Yanming, LI Jianshe, WANG Xiaomin. Comprehensive evaluation of germplasm resources of 100 cherry tomatoes based on different evaluation methods[J]. Journal of South China Agricultural University, 2025, 46(1): 62-71. DOI: 10.7671/j.issn.1001-411X.202401029 |
| [2] | JIA Jia, WANG Huan, DUAN Mingming, CHEN Bo, CHENG Yanbo, NIAN Hai. Accurate identification and correlation analysis of iron and zinc contents in soybean core accessions[J]. Journal of South China Agricultural University, 2023, 44(5): 760-768. DOI: 10.7671/j.issn.1001-411X.202211004 |
| [3] | HU Shuaishuai, WANG Wenzhou, YAN Xiaorong, ZHAO Bohao, ZHU Jie, HAO Ye, CHEN Yang, WU Xinsheng. Myh6 gene expression and its correlation with muscle fiber traits of New Zealand white rabbits[J]. Journal of South China Agricultural University, 2017, 38(2): 12-17. DOI: 10.7671/j.issn.1001-411X.2017.02.003 |
| [4] | ZHOU Guoyan, WU Shaoyun, LONG Wenjie, LEI Yongtao. Drought resistant characters during germination of different wheat germplasm in Yunnan Province and their correlations with the index and coefficient of drought resistance[J]. Journal of South China Agricultural University, 2015, 36(2): 13-18. DOI: 10.7671/j.issn.1001-411X.2015.02.003 |
| [5] | WU Fan, ZHAO Xin, LI Haiyun, ZHANG Jiaen, HU Yinchang, LUO Du, GU Dang’en. Correlation and Difference Analysis to Phenotype Characters for Two Kinds of Pomacea canaliculata[J]. Journal of South China Agricultural University, 2013, 34(2): 254-257. DOI: 10.7671/j.issn.1001-411X.2013.02.024 |
| [6] | WANG Xinian, WANG Jiqing, HU Jiang, LIU Xiu, LI Shaobin, LUO Yuzhu. Correlation Analysis of Polymorphisms of Pit-1 Gene with Production Traits in Goat[J]. Journal of South China Agricultural University, 2013, 34(2): 230-234. DOI: 10.7671/j.issn.1001-411X.2013.02.020 |
| [7] | PAN Shun-shun, LI Jia-xian, SUN Shi-li, ZHAO Chao-yi, HUANG Hua-lin, YAN Chang-yu, LING Cai-jin. Study on Correlation Between Contituents and Liquor Color of a Distinctive Green Tea[J]. Journal of South China Agricultural University, 2012, 33(3): 307-310. DOI: 10.7671/j.issn.1001-411X.2012.03.007 |
| [8] | JIANG Jun-qi1,2,ZHONG Yao-yuan1,LU Yong-yue1,ZENG Ling1,LIANG Guang-wen1. Correlation Analysis on Dynamics of Planthopper Population and Meteorological Factors in Different Resistant Rice Varieties[J]. Journal of South China Agricultural University, 2009, 30(2). DOI: 10.7671/j.issn.1001-411X.2009.02.007 |
| [9] | HUANG Tin-ru1,SUN Yan-wei2,SONG Chang-xu3,CHEN Ping-jie2. Correlation Study on Pseudorabies,Classical Swine Fever,Porcine Reproductive and Respiratory Syndrome,Porcine Circovirus Type 2[J]. Journal of South China Agricultural University, 2009, 30(2). DOI: 10.7671/j.issn.1001-411X.2009.02.024 |
| [10] | HU Yu-kun,ZHAO Qin,Maermila,LI Kai-hui,LU Chun-fang. Comparison experiments and correlated analysis of corn varieties in the middle and lower reaches of Tarm River[J]. Journal of South China Agricultural University, 2004, 25(Z2). |
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: