- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
- Journal of Citation Report of Chinese S&T Journals (Core Edition)
| Citation: |
ZHANG Gongying, YANG Hua, DONG Lihong, WAN Shuqing. Optimization of culture condition of Metarhizium anisopliae for destruxin A production by response surface methodology[J]. Journal of South China Agricultural University, 2018, 39(1): 76-82. DOI: 10.7671/j.issn.1001-411X.2018.01.013
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To raise the destruxin A production of Metarhizium anisopliae by optimizing culture conditions of liquid fermentation.
Experiments were designed by response surface methodology (RSM). The key factors affecting the liquid fermentation were screened by Plackett-Burman design and central composite design.
A model with high fitting degree and small error was obtained. The optimum culture condition of this model was 22.7 g·L–1 sucrose, 13.4 g·L–1 peptone and 9.70 d for fermentation. The maximum predicted concentration of destruxin A was 6.90 μg·mL–1, while the actual measured concentration was 6.89 μg·mL–1.
The results can provide a theoretical basis for large-scale fermentation of M. anisopliae and production of crude toxin, which can potentially enable the widespread development and application of crude toxin.
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