Optimization of culture condition of <i>Metarhizium anisopliae</i> for destruxin A production by response surface methodology

ZHANG Gongying; YANG Hua; DONG Lihong; WAN Shuqing

doi:10.7671/j.issn.1001-411X.2018.01.013

Journal of South China Agricultural University > 2018 > 39(1): 76-82. > DOI: 10.7671/j.issn.1001-411X.2018.01.013

ZHANG Gongying, YANG Hua, DONG Lihong, WAN Shuqing. Optimization of culture condition of Metarhizium anisopliae for destruxin A production by response surface methodology[J]. Journal of South China Agricultural University, 2018, 39(1): 76-82. DOI: 10.7671/j.issn.1001-411X.2018.01.013

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Optimization of culture condition of Metarhizium anisopliae for destruxin A production by response surface methodology

College of Agriculture, South China Agricultural University/Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642, China

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Received Date: April 19, 2017
Available Online: May 17, 2023

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Abstract

Abstract

Objective
To raise the destruxin A production of Metarhizium anisopliae by optimizing culture conditions of liquid fermentation.

Method
Experiments were designed by response surface methodology (RSM). The key factors affecting the liquid fermentation were screened by Plackett-Burman design and central composite design.

Result
A model with high fitting degree and small error was obtained. The optimum culture condition of this model was 22.7 g·L^–1 sucrose, 13.4 g·L^–1 peptone and 9.70 d for fermentation. The maximum predicted concentration of destruxin A was 6.90 μg·mL^–1, while the actual measured concentration was 6.89 μg·mL^–1.

Conclusion
The results can provide a theoretical basis for large-scale fermentation of M. anisopliae and production of crude toxin, which can potentially enable the widespread development and application of crude toxin.
- Metarhizium anisopliae,
- destruxin,
- yield,
- response surface methodology,
- liquid fermentation,
- culture condition

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References (21)

References

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