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    WU Wei, WANG Zuowei, SU Yongying, GAN Shuang, DU Shiping, CAI Yi, GUO Jinya. Analysis of subcellular localization signals of Arabidopsis cytokinin receptor protein AHK3[J]. Journal of South China Agricultural University, 2017, 38(6): 64-71. DOI: 10.7671/j.issn.1001-411X.2017.06.010
    Citation: WU Wei, WANG Zuowei, SU Yongying, GAN Shuang, DU Shiping, CAI Yi, GUO Jinya. Analysis of subcellular localization signals of Arabidopsis cytokinin receptor protein AHK3[J]. Journal of South China Agricultural University, 2017, 38(6): 64-71. DOI: 10.7671/j.issn.1001-411X.2017.06.010
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    Analysis of subcellular localization signals of Arabidopsis cytokinin receptor protein AHK3

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      • Abstract
    • Objective  To study the subcellular localization signals of cytokinin receptors and related mechanisms.
      Method  A series of expression vectors for subcellular localization of Arabidopsis histidine protein kinase 3 (AHK3), one of cytokinin receptor proteins in Arabidopsis, were constructed and transformed into Arabidopsis protoplast cells for transient expression. Laser confocal microscope was used to observe the subcellular localization of AHK3 protein.
      Result  AHK3 is localized in endoplasmic reticulum(ER). Both N and C terminals contain ER localization sequences.
      Conclusion  AHK3 carry out its functions in ER, such as perception of cytokinin, downstream signal transduction and interactions with other receptor proteins, and AHK3 contains multiple ER localization signals.
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