Effects of Chinese herbal formula "Huirukang" on serum E, P4 and TGF-β1 levels of dairy cows during the milk withdrawal period

WU Xiaofeng; XIAO Jinbang; SHEN Liuhong; JIANG Sixun; QIAN Bolin; DENG Junliang; FU Hongqing; ZUO Zhicai; CAO Suizhong; YU Shumin; JIANG Tao

doi:10.7671/j.issn.1001-411X.2017.04.012

Journal of South China Agricultural University > 2017 > 38(4): 69-75. > DOI: 10.7671/j.issn.1001-411X.2017.04.012

WU Xiaofeng, XIAO Jinbang, SHEN Liuhong, JIANG Sixun, QIAN Bolin, DENG Junliang, FU Hongqing, ZUO Zhicai, CAO Suizhong, YU Shumin, JIANG Tao. Effects of Chinese herbal formula "Huirukang" on serum E, P4 and TGF-β1 levels of dairy cows during the milk withdrawal period[J]. Journal of South China Agricultural University, 2017, 38(4): 69-75. DOI: 10.7671/j.issn.1001-411X.2017.04.012

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Effects of Chinese herbal formula "Huirukang" on serum E, P4 and TGF-β1 levels of dairy cows during the milk withdrawal period

1.
Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China
2.
Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China

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Received Date: November 03, 2016
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To explore the impact of Chinese herbal formula "Huirukang" on serum estrogen(E), progesterone(P4) and transforming growth factor β1(TGF-β1) levels in dairy cows during the milk withdrawal period.
Method
Eighty healthy cows in late pregnancy with (15.42±0.71) kg daily milk yield were randomly divided into four groups: control group and Chinese medicine groups(fed 400, 500 and 600 g "Huirukang" daily). The method of gradual milk cessation was used. We collected venous blood from the cow tail on day 0, 1, 3, 5, 7, 9 and 11, and recorded the daily milk yield. ELISA was used to determine the expression levels of serum E, P4 and TGF-β1.
Result
Control group and Chinese medicine groups (400, 500, 600 g) dried off on day 11, 7, 5 and 5 respectively. Serum E levels declined in all four groups, and the decline in control group was slower compared to Chinese medicine groups. P4 level did not change significantly during the milk withdrawal period for control group. P4 levels increased first in Chinese medicine groups, then declined after the milk withdrawal period, and by the end of the test period were still significantly higher compared to control group. TGF-β1 levels increased in all four groups during the milk withdrawal period, and declined after the milk withdrawal period in Chinese medicine groups. The levels of E, TGF-β1 and daily milk yield showed significant correlation between each other during the milk withdrawal period, while P4 had no significant correlation with E, TGF-β1 or daily milk yield.
Conclusion
During the milk withdrawal period, Chinese herbal formula "Huirukang" can significantly promote cows to dry off. E is a negative regulation factor for milk withdrawal, while TGF-β1 is a positive regulation factor. P4 has no significant effect on milk withdrawal.
- cow,
- milk withdrawal period,
- Chinese herbal formula,
- estrogen,
- progesterone,
- TGF-β1

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References (41)

References

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Effects of Chinese herbal formula "Huirukang" on serum E, P4 and TGF-β1 levels of dairy cows during the milk withdrawal period

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