Citation: | LAI Duo, KANG Xianghui, SHAO Xuehua, KUANG Shizi, TIAN Shiyao, XU Hanhong. Effects of neem bioorganic fertilizer on banana growth and fusarium wilt[J]. Journal of South China Agricultural University, 2017, 38(4): 30-36. DOI: 10.7671/j.issn.1001-411X.2017.04.005 |
To manufacture neem bioorganic fertilizer using neem cake waste, and test its effects on banana growth and fusarium wilt caused by Fusarium oxysporum f. sp. cubense.
Neem bioorganic fertilizer was prepared by fermenting neem cake with a biocontrol bacteria Bacillus amyloliquefaciens HN-11. The effects of neem bioorganic fertilizer on banana growth and fusarium wilt were determinated by inhibition test and pot experiment. The pathogen mycelial morphology was observed by using a scanning electron microscope (SEM).
B.amyloliquefaciens HN-11 had obvious inhibitory activity against F. oxysporum Foc4 with an inhibition rate of 72.1%. Biocontrol strain HN-11 had good compatibility with neem cake. Treated with 5% and 10% neem bioorganic fertilizer, the banana grew better than the control group with the fresh weight, dry mass, plant height and stem diameter significantly increasing. The disease indexes of the two treatment groups were 20 and 25, and the control efficiencies against fusarium wilt disease reached 72.2% and 77.8%, respectively. Strain HN-11 reduced the number of Foc4 spores in soil and destroyed their mycelial structure.
Application of mature neem cake by fermenting with Bacillus amyloliquefaciens HN-11 could avoid phytotoxicity to banana plants. The prepared neem bioorganic fertilizer can efficiently promote banana growth and prevent fusarium wilt disease.
[1] |
WU Y L, YI G J, PENG X X, et al. Systemic acquired resistance in Cavendish banana induced by infection with an incompatible strain of Fusarium oxysporum f. sp. cubense[J]. J Plant Physiol, 2013, 170(11): 1039-1046. doi: 10.1016/j.jplph.2013.02.011
|
[2] |
杨江舟, 张静, 胡伟, 等.韭菜根系浸提液对香蕉枯萎病和土壤微生物生态的影响[J].华南农业大学学报, 2012, 33(4): 480-487. http://www.cnki.com.cn/Article/CJFDTOTAL-HNNB201204012.htm
|
[3] |
张志红, 李华兴, 韦翔华, 等.生物肥料对香蕉枯萎病及土壤微生物的影响[J].生态环境, 2008, 17(6): 2421-2425. http://www.cnki.com.cn/Article/CJFDTOTAL-TRYJ200806061.htm
|
[4] |
匡石滋, 李春雨, 田世尧, 等.药肥两用生物有机肥对香蕉枯萎病的防治及其机理初探[J].中国生物防治学报, 2013, 29(3): 417-423. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201303014.htm
|
[5] |
肖相政, 刘可星, 廖宗文.生物有机肥对番茄青枯病的防效研究及机理初探[J].农业环境科学学报, 2009, 28(11): 2368-2373. doi: 10.3321/j.issn:1672-2043.2009.11.028
|
[6] |
ZHANG S S, RAZA W, YANG X M, et al. Control of fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer[J]. Biol Fertil Soils, 2008, 44:1073-1080. doi: 10.1007/s00374-008-0296-0
|
[7] |
何欣, 郝文雅, 杨兴明, 等.生物有机肥对香蕉植株的生长和香蕉枯萎病防治的研究[J].植物营养与肥料学报, 2010, 16(4): 978-985. doi: 10.11674/zwyf.2010.0430
|
[8] |
张志红, 冯宏, 肖相政, 等.生物肥防治香蕉枯萎病及对土壤微生物多样性的影响[J].果树学报, 2010, 27(4): 575-579. http://www.cnki.com.cn/Article/CJFDTOTAL-GSKK201004019.htm
|
[9] |
YUAN J, RUAN Y Z, WANG B B, et al. Plant growth-promoting rhizobacteria strain Bacillus amyloliquefaciens NJN-6-enriched bio-organic fertilizer suppressed fusarium wilt and promoted the growth of banana plants[J]. J Agric Food Chem, 2013, 61(16): 3774-3780. doi: 10.1021/jf400038z
|
[10] |
FU L, RUAN Y Z, TAO C Y, et al. Continous application of bioorganic fertilizer induced resilient culturable bacteria community associated with banana fusarium wilt suppression[J]. Sci Rep, 2016, 6: 27731. doi: 10.1038/srep27731
|
[11] |
李国良, 姚丽贤, 杨苞梅, 等.有机肥与生防菌结合防治香蕉枯萎病的初步研究[J].中国土壤与肥料, 2012(2): 67-72. http://www.cnki.com.cn/Article/CJFDTOTAL-TRFL201202014.htm
|
[12] |
许志刚.普通植物病理学[M].北京:中国农业出版社, 2002: 236-262.
|
[13] |
SARAVANAN T, MUTHUSAMY M, MARIMUTHU T. Effect of Pseudomonas fluorescens on Fusarium wilt pathogen in banana rhizosphere[J]. J Biol Sci, 2004, 4(2): 192-198. doi: 10.3923/jbs.2004.192.198
|
[14] |
蔡燕飞, 廖宗文, 章家恩, 等.生态有机肥对番茄青枯病及土壤微生物多样性的影响[J].应用生态学报, 2003, 14(3): 349-353. http://www.cnki.com.cn/Article/CJFDTOTAL-YYSB200303006.htm
|
[15] |
SINGH U P, SINGH H B, SINGH R B. The fungicidal effect of neam (Azadirachta indica) extract on some soil borne pathogen of gram (Cicer arietinum)[J]. Mycologia, 1980, 72(6): 1077-1093. doi: 10.2307/3759561
|
[16] |
SARAVANAN T, MUTHUSAMY M, MATIMUTHU T. Development of integrated approach to manage the fusarial wilt of banana[J]. Crop Prot, 2003, 22 (9): 1117-1123. doi: 10.1016/S0261-2194(03)00146-7
|
[17] |
KUMAR D, SINGH K P, JAISWAL R K. Effect of fertilizers and neem cake amendment in soil on spore germination of Arthrobotrys dactyloides[J]. Mycobiology, 2005, 33(4): 194-199. doi: 10.4489/MYCO.2005.33.4.194
|
[18] |
赵欢欢, 叶仲辉, 杨文杰, 等.茶枯对香蕉生长和香蕉枯萎病的影响[J].西南大学学报(自然科学版), 2015, 37(9): 25-30. http://www.cnki.com.cn/Article/CJFDTOTAL-XNND201509004.htm
|