Genetic diversity of Zenia insignis based on SRAP markers

Objective To study population genetic diversity of Zenia insignis, and to provide a basis for Z. insignis germplasm protect and promote genetic improvement.

Method Base on establishing of SRAP-PCR system in Z. insignis, the genetic diversity among 17 provenances was analyzed. UPGMA clustering analysis was used to divide Z. insignis provenances into different groups.

Result A total of 151 bands were amplified from 12 primer pairs, and in average 12.58 bands were amplified from each primer pair. There were 106 polymorphic bands, in average 8.83 bands per primer sets, and the average percentage of polymorphic bands was 70.39%. The ratios of polymorphic loci among provenances were 38.96%-72.73%, and 59.66% in average. The genetic diversity indices were 0.175 5-0.313 3, and 0.256 8 in average. The Shannon information indices were 0.249 4-0.450 2, and 0.369 1 in average. The numbers of alleles (na) observed were 1.519 5-1.727 3, and 1.600 0 in average. The number of alleles (na) at the provenance level was 1.724 9. The numbers of effective alleles (ne) were 1.330 5-1.577 3, and 1.4713 in average. The number of effective alleles (ne) at the provenance level was 1.502 6. The genetic identity degrees among provenances were 0.703 1-0.886 5.The genetic distances were 0.120 5-0.352 3. According to cluster analysis, 17 provenances were divided into three groups. The first group included Guangxi and Guizhou provenances. The second group included Guangdong, Hunan and Guangxi provenances. The third group only included Yunnan provenance. The provenances with geographic proximity were generally clustered into the same group.

Conclusion The genetic diversity is abundant among Z. insignis provenances and among individuals within provenance, but is mainly from individuals within provenance. Therefore more attention should be paid to individuals in genetic improvement of Z. insignis. Both the low level of gene flow among provenances and three clear geographic clustering should be caused by the geographic isolation due to the specific living environment of Z.insignis.