Effect of drought stress on leaf protective enzyme activities, contents of osmoregulation substances and quality of <i>Saposhnikovia divaricata</i>

HAN Zhongming; XU Miaomiao; WANG Yunhe; ZHANG Tao; HAN Mei; YANG Limin

doi:10.7671/j.issn.1001-411X.2016.06.014

Journal of South China Agricultural University > 2016 > 37(6): 91-97. > DOI: 10.7671/j.issn.1001-411X.2016.06.014

HAN Zhongming, XU Miaomiao, WANG Yunhe, ZHANG Tao, HAN Mei, YANG Limin. Effect of drought stress on leaf protective enzyme activities, contents of osmoregulation substances and quality of Saposhnikovia divaricata[J]. Journal of South China Agricultural University, 2016, 37(6): 91-97. DOI: 10.7671/j.issn.1001-411X.2016.06.014

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Effect of drought stress on leaf protective enzyme activities, contents of osmoregulation substances and quality of Saposhnikovia divaricata

College of Chinese Medicinal Materials, Jilin Agricultural University/Key Laboratory for Ecological Restoration and Ecosystem Management of Jilin Province, Changchun 130118, China

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Received Date: February 17, 2016
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To investigate the physiological mechanisms of drought adaptation and protective enzyme system of Saposhnikovia divaricata under drought stress.
Method
Three levels of water supply including full irrigation (CK), light drought stress(LD) and severe drought stress (SD) treatments were set up for annual S. divaricata. Effects of drought stress on leaf protective enzyme activities, contents of osmoregulation substances and total content of two chromones (prim-O-glucosylcimifugin and 5-O-methylvisammioside) of S. divaricata were studied.
Result
The activities of superoxyde dismutase (SOD), peroxidase (POD), catalase (CAT), and proline content under different water treatments increased during early test stage, reached peak during middle stage and then decreased. Malondialdehyde (MDA) content under different water treatments increased throughout the entire test period. The total contents of two chromones under different water treatments all increased at early stage, reached peak during middle stage and then decreased for LD and SD treatments, while slowly increased during both middle and late stage for CK treatment. Changes in the activities of SOD, POD and CAT, the contents of osmoregulation substances, and the total contents of two chromones for LD and SD treatments were higher compared to CK, and the peak values of these measurements for LD and SD treatments were also higher compared to CK. There were significant correlations between CAT activity, the total content of two chromones and SOD activity under different water treatments.
Conclusion
Appropriately imposing drought stress in the cultivation of S. divaricata can increase protective enzyme activities and the contents of osmoregulation substances of S. divaricata leaves, which is beneficial for S. divaricata growth and can increase the total content of two chromones.
- Saposhnikovia divaricata,
- drought stress,
- protective enzyme,
- proline,
- MDA,
- chromone

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Effect of drought stress on leaf protective enzyme activities, contents of osmoregulation substances and quality of Saposhnikovia divaricata

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