Screening two entomopathogenic fungal strains with high virulence against Asia citrus psyllid, <i>Diaphorina citri</i>

DAI Xiaoyan; LI Yihan; XU Weiming; SHEN Zule; HUANG Zhen; WU Jianhui; REN Shunxiang; QIU Baoli

doi:10.7671/j.issn.1001-411X.2016.05.011

Journal of South China Agricultural University > 2016 > 37(5): 62-65. > DOI: 10.7671/j.issn.1001-411X.2016.05.011

DAI Xiaoyan, LI Yihan, XU Weiming, SHEN Zule, HUANG Zhen, WU Jianhui, REN Shunxiang, QIU Baoli. Screening two entomopathogenic fungal strains with high virulence against Asia citrus psyllid, Diaphorina citri[J]. Journal of South China Agricultural University, 2016, 37(5): 62-65. DOI: 10.7671/j.issn.1001-411X.2016.05.011

Citation:

PDF (413 KB)

Screening two entomopathogenic fungal strains with high virulence against Asia citrus psyllid, Diaphorina citri

1.
College of Agriculture, South China Agricultural University/Key Laboratory of Bio-Pesticide Innovation and Application/ Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, Guangzhou 510640, China
2.
Plant Protection Institute, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China

More Information

Received Date: January 13, 2016
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To screen entomopathogenic fungal strains with high virulence against Asia citrus psyllid (ACP), Diaphorina citri Kuwayama, and to provide a basis for development of microbial pesticide against ACP.
Method
The growth and sporulation rates of five Beauveria bassiana strains numbered A to E and five Isaria fumosorosea strains numbered A to E, and the mortality rates of ACP after infected by these ten fungal strains were investigated.
Result
The spores quantity of five B. bassiana strains were significantly different after cultured for 10 days, strain E had the largest spores quantity(3.78×10⁸ mL^-1) and strain A had the least spores quantity(7.96×10⁷ mL^-1). There was significant difference of spores quantity between strain E and strain A. There was no significant difference among the mortality rates of 2-3 instar ACP nymphs seven days after the spray of 1×10⁶ mL^-1 B. bsssiana fungal spores, and the highest mortality rate (78.0%) was found in the strain A treatment, followed by the strain E treatment (77.0%). For five I. fumosorosea strains, the spores quantity after cultured for 10 days, strain B had the highest sporulation rate (6.04×10⁸ mL^-1) and strain E had the least spores quantity(6.28×10⁷ mL^-1). There was significant difference of spores quantity between strain B and strain E. The mortality rates of 2-3 instar ACP nymphs seven days after the spray of 1×10⁶ mL^-1 I. fumosorosea fungal spores were higher than the control group except strain D. ACP infected by strain B had the highest mortality rate(81.0%).
Conclusion
B. bassiana strain E and I. fumosorosea strain B are considered with high virulence against ACP and can be potentially used for biological control of ACP.
- Diaphorina citri,
- Beauveria bassiana,
- Isaria fumosorosea,
- pathogenicity,
- biological control

FullText(HTML)

References (16)

References

[1]	黄金萍, 黄建邦, 高娃, 等.柑橘木虱取食黄龙病柑橘部位与获菌效率的关系[J].华南农业大学学报, 2015, 36(1):71-74. http://xuebao.scau.edu.cn/zr/hnny_zr/ch/reader/view_abstract.aspx?flag=1&file_no=201501015&journal_id=hnny_zr
[2]	代晓彦, 任素丽, 周雅婷, 等.黄龙病媒介昆虫柑橘木虱生物防治新进展[J].中国生物防治学报, 2014, 30(3): 414-419. http://d.old.wanfangdata.com.cn/Periodical/zgswfz201403022
[3]	邓明学.以控制木虱为重点的柑橘黄龙病综合防治技术研究[J].植物保护, 2006, 32(6): 147-149. doi: 10.3969/j.issn.0529-1542.2006.06.044
[4]	刘宗庆.桂北柑桔黄龙病发生流行及综合治理[J].广西园艺, 2006, 17(4): 6-7. doi: 10.3969/j.issn.1674-5868.2006.04.002
[5]	叶志勇, 於一敏, 余继华.柑橘木虱的发生及综合防治技术[J].浙江柑橘, 2006, 23(3): 28-30. doi: 10.3969/j.issn.1009-0584.2006.03.015
[6]	熊红利, 林云彪, 项宇, 等.柑橘黄龙病与柑橘木虱在我国发生北界调查[J].植物检疫, 2011, 25(4): 79-80. http://d.old.wanfangdata.com.cn/Periodical/zwjy201104023
[7]	杜丹超, 鹿连明, 胡秀荣, 等.淡紫紫孢菌菌株的分离、鉴定及其对柑橘木虱的致病性[J].浙江农业学报, 2015, 27(3): 393-399. doi: 10.3969/j.issn.1004-1524.2015.03.13
[8]	章玉苹, 李敦松, 黄少华, 等.柑橘木虱的生物防治研究进展[J].中国生物防治, 2009, 25(2): 160-164. doi: 10.3321/j.issn:1005-9261.2009.02.013
[9]	GOTTWALD T R. Current epidemiological understanding of citrus Huanglongbing[J]. Annu Rev Phytopathol, 2010, 48: 119-139. doi: 10.1146/annurev-phyto-073009-114418
[10]	HALBERT S E, MANJUNATH K L. Asian citrus psyllid (Sternorryncha: Psyllidae) and greening disease of citrus: A literature review and assessment of risk in Florida[J]. Fla Entomol, 2004, 87(3):330-353. doi: 10.1653/0015-4040(2004)087[0330:ACPSPA]2.0.CO;2
[11]	YANG Y, HUANG M, BEATTIE G A, et al. Distribution, biology, ecology and control of the psyllid Diaphorina citri Kuwayama, a major pest of citrus: Astatus report for China[J]. Int J Pest Manage, 2006, 52(4): 343-352. doi: 10.1080/09670870600872994
[12]	HALBERT S E, NUNEZ C A. Distribution of the Asian citrus psyllid, Diaphorina citri Kuwayama (Rhynchota: Psyllidae) in the Caribbean basin[J]. Fla Entomol, 2004, 87(3): 401-402. doi: 10.1653/0015-4040(2004)087[0401:DOTACP]2.0.CO;2
[13]	BOVE J M. Huanglongbing: A destructive, newly-emerging, century-old disease of citrus[J]. J Plant Pathol, 2006, 88(1): 7-37. http://d.old.wanfangdata.com.cn/Periodical/zgnxtb201004052
[14]	杜丹超, 尤明其, 胡秀荣, 等.柑橘木虱致病真菌淡紫拟青霉ZJPL08的生物学特性研究[J].浙江柑橘, 2014, 31(4):17-21. http://d.old.wanfangdata.com.cn/Periodical/zjgj201404007
[15]	SUBANDIYAH S, NIKOH N, SATO H, et al. Isolation and characterization of two entomopathogenic fungi attacking Diaphorina citri (Homoptera, Psylloidea) in Indonesia[J]. Mycoscience, 2000, 41(5): 509-513. doi: 10.1007/BF02461672
[16]	HOY M A, SINGH R, ROGERS M E. Evaluations of a novel isolate of Isaria fumosorosea for control of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae)[J]. Fla Entomol, 2010, 93(1): 24-32. doi: 10.1653/024.093.0103

Cited By

Get Citation

PDF

XML

Article views (1833) PDF downloads (1917)

Turn off MathJax

Article Contents

Abstract

References

Screening two entomopathogenic fungal strains with high virulence against Asia citrus psyllid, Diaphorina citri

Abstract

References

Catalog

Export File

Citation

Format

Content