| Citation: |
DENG Zhenshan, ZHANG Weimin, CHEN Miao. Screening fungal endophytes from apple trees and their antagonistic characteristics[J]. Journal of South China Agricultural University, 2016, 37(2): 73-78. DOI: 10.7671/j.issn.1001-411X.2016.02.011
|
To screen fungal endophytes against Valsa malimiyabe and Glomeralla cingulata from apple trees and explore their inhibitory effects.
Fungal endophytes were isolated from roots, stems and rhizosphere soil of healthy apple plants. Their antifungal activities against V. malimiyabe and G. cingulata were examined using in vitro dual culture assay. Heat-resistant properties of antifungal substances were tested using the fermentation culture of the isolated fungal endophytes.
Eighteen endophytic fungus strains belonging to three genera were isolated and half of the 18 strains were from stem. The dual culture assay showed eight strains had above 60% inhibition rate against V. malimiyabe and G. cingulata. J2, J16 and J17 strains strongly inhibited V. malimiyabe with the inhibition rates of 82.75%, 84.31% and 82.35% respectively. The inhibition rate of T1 to G. cingulata reached 80.67%. The culture filtrates of fungal endophytes J2, J16, J17, J24, T1 inhibited the conidia germination of both pathogens, and all of the inhibition rates were not less than 39.55% and the highest value was 69.75%. The sterile filtrate of J11 and T1 showed high heat stability at 121 ℃, while J2 lost bioactivity at 100 ℃.
Fungal endophytes J2, J11, J16, J17 and T1 have strong inhibitory effects against V. malimiyabe and G. cingulata, and therefore are potential candidates for biological control of these two pathogens.
| [1] |
刘如香. 苹果树腐烂病菌分生孢子的萌发、存活和侵染条件的研究[D]. 保定: 河北农业大学, 2011.
|
| [2] |
李培夫.苹果腐烂病防治新技术[J].农村科技, 2005(4): 17. doi: 10.3969/j.issn.1002-6193.2005.04.013
|
| [3] |
杨万宝.苹果炭疽病发生原因及防治措施[J].现代农村科技, 2011(19): 26. doi: 10.3969/j.issn.1674-5329.2011.19.021
|
| [4] |
王敬尊.苹果炭疽病的识别与防治[J].安徽农业科学, 2009, 37(18): 8560-8561. doi: 10.3969/j.issn.0517-6611.2009.18.105
|
| [5] |
邓振山, 白重炎, 李军, 等.陕北苹果常见真菌病害病原菌的分离鉴定研究[J].延安大学学报(自然科学版), 2006, 25 (4):65-70. doi: 10.3969/j.issn.1004-602X.2006.04.019
|
| [6] |
李培, 王永斌.植物内生真菌的研究与展望[J].饮料工业, 2008, 11(7): 11-13. doi: 10.3969/j.issn.1007-7871.2008.07.004
|
| [7] |
AZEVEDP J L, MACCHERONI J R W, PEREIRA J O, et al. Endophytic microorganisms: A review on tropical plants[J]. Electron J Biotechn, 2000, 3(1): 40-65.
|
| [8] |
王现坤, 张晓华, 赫双红.侧柏内生真菌的分离鉴定及抗菌活性筛选[J].农药, 2010, 49(7): 519-521. doi: 10.3969/j.issn.1006-0413.2010.07.018
|
| [9] |
蔡光华, 战峰, 王晓玲.苹果树腐烂病拮抗内生细菌PG-10-8-11v菌株抗菌物质的初步研究[J].西南民族大学学报(自然科学版), 2011, 37(6): 945-949. http://d.old.wanfangdata.com.cn/Periodical/xnmzxyxb201106019
|
| [10] |
邓振山, 赵龙飞, 张薇薇, 等.银杏内生真菌的分离及其对苹果腐烂病病原菌的拮抗作用[J].西北植物学报, 2009, 29(3): 608-613. doi: 10.3321/j.issn:1000-4025.2009.03.028
|
| [11] |
郭建新, 孙广宇, 张荣, 等.银杏内生真菌抗真菌活性菌株的分离和筛选[J].西北农业学报, 2005, 14(4): 14-17. doi: 10.3969/j.issn.1004-1389.2005.04.004
|
| [12] |
徐涛, 胡同乐, 王亚南, 等.苹果树皮内生真菌的分离及其对腐烂病的生物防治潜力[J].植物保护学报, 2012, 39 (4): 327-333. http://d.old.wanfangdata.com.cn/Periodical/zwbhxb201204007
|
| [13] |
周宗山, 徐成楠, 吴玉星, 等.苹果树腐烂病病菌分生孢子获取方法研究[J].中国果树, 2011(2): 41-43. http://d.old.wanfangdata.com.cn/Periodical/zhongggs201102015
|
| [14] |
杨润亚, 李艳华, 柳娜娜.连翘内生真菌的分离及其抑菌活性初步研究[J].安徽农业科学, 2007, 35 (15): 4561-4563. doi: 10.3969/j.issn.0517-6611.2007.15.085
|
| [15] |
夏伟, 张红, 颜艳伟, 等.棘孢木霉L4对立枯丝核菌的拮抗机制[J].植物保护学报, 2010, 37 (5): 477-478. http://d.old.wanfangdata.com.cn/Periodical/zwbhxb201005019
|
| [16] |
孙杰, 杨润亚, 任晓明.三种海藻内生真菌的分离及其抑菌活性研究[J].食品科学, 2007(11): 357-359. doi: 10.3321/j.issn:1002-6630.2007.11.082
|
| [17] |
王艳红, 吴晓民, 朱艳萍, 等.温郁金内生真菌Chaetomium globosum L18对植物病原菌的抑菌谱及拮抗机理[J].生态学报, 2012, 32(7): 2040-2046.
|
| [18] |
蒋继宏, 李晓储, 陈凤美, 等.植物抽提物对苹果炭疽病菌分生孢子的抑制作用[J].江苏林业科技, 2004, 31 (4): 23-25. doi: 10.3969/j.issn.1001-7380.2004.04.006
|
| [19] |
邓振山, 候改成, 孙志宏, 等.番茄灰霉病菌拮抗菌D6和D10发酵条件的优化及其抑菌效果[J].西北农林科技大学学报(自然科学版), 2013, 41 (9): 73-77. http://d.old.wanfangdata.com.cn/Periodical/xbnydxxb201309013
|
| [20] |
展丽然. 苹果腐烂病菌拮抗放线菌的筛选、鉴定及发酵条件的优化[D]. 保定: 河北农业大学, 2008.
|
| [21] |
王东昌, 辛玉成, 郝秀青, 等.苹果树枝干病害的生物防治[J].吉林农业科学, 2001, 26 (2): 49-50. doi: 10.3969/j.issn.1003-8701.2001.02.013
|
| [22] |
XIN Y F, SHANG J J. Bio-control trials of Chaetominum spirale ND35 against apple canker[J]. J Forest Res-Jpn, 2005, 16 (2): 121-124. doi: 10.1007/BF02857904
|
| [23] |
郭晓, 高克祥, 印敬明, 等.螺旋毛壳ND35 β-1, 3-葡聚糖酶的诱导、性质及其抑菌作用[J].植物病理学报, 2005, 35(6): 493-503. doi: 10.3321/j.issn:0412-0914.2005.06.003
|
| [1] | SHENG Qingqing, ZHAO Chen, YAN Ying, XU Hanhong. Synthesis and bioactivity evaluation of novel urea derivatives of arylpyrazoles[J]. Journal of South China Agricultural University, 2018, 39(2): 75-81. DOI: 10.7671/j.issn.1001-411X.2018.02.012 |
| [2] | HUANG Xian-pei,LUO Jian-jun,HU Mei-ying,LIANG Ying-yi,YE Xin-xin,ZHONG Guo-hua. Isolation and Bioactivity of Endophytic Fungi in Derris hancei[J]. Journal of South China Agricultural University, 2009, 30(2). DOI: 10.7671/j.issn.1001-411X.2009.02.012 |
| [3] | YUE Guang-fang,CHEN Yi-tian,LIU Ying,LI Wan-li,SHI Zhen-dan. Preparation of Bioactive Recombinant Chicken Prolactin[J]. Journal of South China Agricultural University, 2009, 30(1). DOI: 10.7671/j.issn.1001-411X.2009.01.019 |
| [4] | WAN Shu-qing,XU Han-hong,ZHAO Shan-huan,LIU Qing-xin. Study on Bioactivity of Polyacetylene Compounds to Pieris rapae[J]. Journal of South China Agricultural University, 2007, 28(2): 56-60. DOI: 10.7671/j.issn.1001-411X.2007.02.014 |
| [5] | CHEN Huan-yu,FENG Xia,L Li-hua,LI Shu-quan,CHEN Wei-hong. Study on Physical and Chemical Properties of Avermectin Preparations and Their Bioactivity to Plutella xylostella[J]. Journal of South China Agricultural University, 2006, 27(3): 44-47. DOI: 10.7671/j.issn.1001-411X.2006.03.012 |
| [6] | LI Yong-jun,ZHANG Mao-xin,GU Wen-xiang,XIAN Guo-liang. Preparation of Neem Oil Microcapsule and Its Bioactivity[J]. Journal of South China Agricultural University, 2006, 27(2): 53-55. DOI: 10.7671/j.issn.1001-411X.2006.02.015 |
| [7] | Study on the Bioactivities of Neem Seed Extracts to Green Leafhoppers[J]. Journal of South China Agricultural University, 2003, 24(3): 30-32. DOI: 10.7671/j.issn.1001-411X.2003.03.008 |
| [8] | Ali Al-Bashari, Zhong Guohua, Hu Meiying. Effects on the Bioactivity of the Extracts from Myoporum bontioides Against Imported Cabbage Worm (Pieris rapae)[J]. Journal of South China Agricultural University, 1999, (3): 28-31. |
| [9] | Wang Wenlu Chiu Shin -Foon. STUDIES ON THE INSECTICIDAL PRINCIPLES AND BIOLOGICAL ACTIVITIES OF MELIA AZEDARACH L.[J]. Journal of South China Agricultural University, 1993, (3): 64-69. |
| [10] | Zhang YeGuang Chiu Yutong Chiu ShinFoon. PRILIMINARY STUDIES ON THE BIOACTIVITY OF THE EXTRACTS FROM AJUGA NIPPONENSIS MAKINO AGAINST FOUR SPECIES OF LEPIDOPTEROUS INSECT PESTS[J]. Journal of South China Agricultural University, 1992, (4): 63-68. |
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: