| Citation: |
HAN Ying, XIN Xiaotong, HAN Xiaori, LI Jun, LI Jiaqi. Effects of different long-term fertilization patterns on soil microflora[J]. Journal of South China Agricultural University, 2016, 37(2): 51-58. DOI: 10.7671/j.issn.1001-411X.2016.02.008
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To study effects of different fertilization treatments on soil microbial number, microbial biomass carbon(MBC), microbial biomass nitrogen(MBN) and microbial biomass phosphorus(MBP).
Fifteen soil samples were collected from the long-term located fertilization experimental station of Shenyang Agricultural University. The effects of fertilization on microbial number and microbial biomass, and the correlation were studied using microbial culture and chloroform extraction method.
Fertilization had a positive effect on microbial number and microbial biomass. The most affected soil microbes were bacteria, followed by fungi and actinomycetes.The most affected microbial biomass was MBP, followed by MBN and MBC. Among 15 treatments, organic treatment M1 had the highest number of bacteria, organic treatment M2 had the highest number of actinomycetes, and M1NPK treatment combining organic and inorganic fertilizers had the highest number of fungi. M1 had the highest number of MBC and MBN, while M2NPK with organic and inorganic fertilizers had the highest number of MBP. There was a positive correlation between MBC and microbial number.
Organic fertilizer significantly increases microbial number. Reducing inorganic fertilizer can increase MBC and MBN with organic and inorganic fertilizers. Appropriate application of nitrogen and phosphorus fertilizers can promote the microbe to absorb nitrogen and phosphorus, and significantly increase MBN and MBP. Nitrogen fertilizer application can accelerate the loss of soil fertility which causes the soil to change from bacteria type to fungi type. Organic fertilizer application can increase soil fertility.
| [1] |
李阜棣.土壤微生物学[M].北京:中国农业出版社, 1996.
|
| [2] |
姚槐应, 黄昌勇.土壤微生物生态学及其实验技术[J].北京:科学出版社, 2006:5-6.
|
| [3] |
樊军, 郝明德.长期轮作与施肥对土壤主要微生物类群的影响[J].水土保持研究, 2003, 10(1):88-114. doi: 10.3969/j.issn.1005-3409.2003.01.026
|
| [4] |
顾美英, 徐万里, 茆军, 等.连作对新疆绿洲棉田土壤微生物数量及酶活性的影响[J].干旱地区农业研究, 2009, 27(1):1-5. http://d.old.wanfangdata.com.cn/Periodical/ghdqnyyj200901001
|
| [5] |
林葆, 林继雄, 李家康.长期施肥的作物产量和土壤肥力变化[M].北京:中国农业科技出版社, 1996:1-179.
|
| [6] |
王光华, 齐晓宁, 金剑, 等.施肥对黑土农田土壤全碳、微生物量碳及土壤酶活性的影响[J].土壤通报, 2007, 38(4):661-665. doi: 10.3321/j.issn:0564-3945.2007.04.009
|
| [7] |
INSAM H, MITCHELL C C, DORMAAR J F. Relationship of soi1 mierobial biomass and activity with fertilization practice and crop yield of three ultisols[J]. Soil Biol Biochem, 1991, 23(5):459-464. doi: 10.1016/0038-0717(91)90010-H
|
| [8] |
LEIGH R A, JOHNSTON A E. Long-term experiments in agricultural and ecological sciences[C]// Proceeding of a conference to celebrate the 150th anniversary of rothamsted experimental station. Cambridge: the UK at University Press, 1994: 14-17.
|
| [9] |
赖庆旺, 李茶苟, 黄庆海.红壤性水稻土无机肥连施与土壤结构特性的研究[J].土壤学报, 1992, 29(2):168-185. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000003130439
|
| [10] |
沈善敏.国外的长期肥料试验:(一)[J].土壤通报, 1984, 15(2):85-91.
|
| [11] |
沈善敏.国外的长期肥料试验:(二)[J].土壤通报, 1984, 15(2):134-138.
|
| [12] |
沈善敏.土壤科学与农业持续发展[J].土壤学报, 1994, 31(2):113-118. http://d.old.wanfangdata.com.cn/Periodical/hnny201804019
|
| [13] |
沈善敏.长期土壤肥力试验的科学价值[J].植物营养与肥料学报, 1995, 1(1):1-7. doi: 10.3321/j.issn:1008-505X.1995.01.001
|
| [14] |
林新坚, 王飞, 蔡海松, 等.不同有机肥源对土壤微生物生物量及花生产量的影响[J].中国生态农业学报, 2009, 17(2):235-238. http://d.old.wanfangdata.com.cn/Periodical/stnyyj200902007
|
| [15] |
何晓雁, 郝明德, 李慧成, 等.黄土高原旱地小麦施肥对产量及水肥利用效率的影响[J].植物营养与肥料学报, 2010, 16(6):1333-1340. http://d.old.wanfangdata.com.cn/Periodical/zwyyyflxb201006006
|
| [16] |
徐明岗, 梁国庆, 张夫道.中国土壤肥力演变[M].北京:中国农业科学出版社, 2006.
|
| [17] |
DICKR P, RASMUSSEN P E, KERLE E A. Influence of long-term residue management on soil enzyme activity in relation to soil chemical properties of a wheat-fallow system[J]. Biol Fert Soils, 1988, 6(2):159-164. doi: 10.1007-BF00257667/
|
| [18] |
ELFSTRAND S, HEDLUND K, MARTENSSON A. Soil enzyme activities, microbial community composition and function after 47 years of continuous green manuring[J]. Appl Soil Ecol, 2007, 35(3):610-621. doi: 10.1016/j.apsoil.2006.09.011
|
| [19] |
PETRA M, ELLEN K, BERND M. Structure and function of the soil microbial community in a long-term fertilizer experiment[J]. Soil Biol Biochem, 2003, 35(3):453-461. doi: 10.1016/S0038-0717(02)00297-3
|
| [20] |
赵立勇, 韩晓日, 杨劲峰, 等, 长期定位施肥对作物产量及土壤有效养分的影响[J].杂粮作物, 2008, 28(1):45-48. doi: 10.3969/j.issn.2095-0896.2008.01.020
|
| [21] |
庄岩, 吴凤芝, 杨阳, 等.轮套作对黄瓜土壤微生物多样性及产量的影响[J].中国农业科学, 2009, 42(1):204-209. doi: 10.3864/j.issn.0578-1752.2009.01.025
|
| [22] |
李秀英, 赵秉强, 李絮花, 等.不同施肥制度对土壤微生物的影响及其与土壤肥力的关系[J].中国农业科学, 2005, 38(8):1591-1599. doi: 10.3321/j.issn:0578-1752.2005.08.014
|
| [23] |
封海胜, 万书波, 左学青, 等.花生连作土壤及根际主要微生物类群的变化及产量的相关[J].花生科技, 1999(增刊):277-283. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199900515170
|
| [24] |
王继红, 刘景双, 于君宝, 等.氮磷肥对黑土玉米农田生态系统土壤微生物量碳、氮的影响[J].水土保持, 2004, 18(1):35-38. http://d.old.wanfangdata.com.cn/Periodical/trqsystbcxb200401009
|
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