Objective This study aimed to lay a theoretical foundation for cold resistance breeding and to provide a technical support for artificial cultivation of Neolamarckia cadamba.
Method After the branches of seven provenances were treated with artificially induced low temperature, the contents of methane dicarboxylic aldehyde(MDA), free proline, soluble sugar and soluble protein, the relative electrical conductivity (REC) as well as the branch recovery growth were measured. Using five indicators suitable for determination of cold resistance, combined with principal component analysis and membership function method, the cold resistance of different provenances was comprehensively evaluated.
Result Under artificially induced low temperature stress(-2 ℃), the Longzhou provenance had the highest MDA content(35.24 μg·g-1) and Baoshan had the lowest(21.19 μg·g-1); Longzhou had the highest proline content (9.98 μg·g-1) and Dehong had the lowest(6.13 μg·g-1); Yunfu had the highest content of soluble sugar(79.66 μg·g-1) and Jinghong had the lowest(65.32 μg·g-1); Tianhe had the highest content of soluble protein(24.89 μg·g-1) and Jinghong had the lowest(19.57 μg·g-1); Baoshan had the highest REC (58.27%) and Yunfu had the lowest(48.37%); During the branch recovery growth, Yunfu had the highest branch bud survival rate(42.03%) and Jinghong had the lowest(3.88%). The integrated indices of cold resistance for seven provenances, Yunfu, Longzhou, Tianhe, Xingning, Jinghong, Baoshan and Dehong were 0.951, 0.863, 0.755, 0.728, 0.191, 0.151 and 0.132 respectively.
Conclusion The cold resistance of Yunfu provenance was the strongest, followed by Longzhou, Tianhe, Xingning, Jinghong, Baoshan and Dehong. In general, the cold resistance of Guangdong or Guangxi provenance was stronger than that of Yunnan provenance.
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