- Chinese Core Journal
- Chinese Science Citation Database (CSCD) Source journal
- Journal of Citation Report of Chinese S&T Journals (Core Edition)
| Citation: |
CHEN Lijun, LIU Mingqian, LIAO Boyong, LI Juncheng, CHEN Xiaoyang. Analysis of genetic diversity of Melia azedarach with SRAP markers[J]. Journal of South China Agricultural University, 2016, 37(1): 70-74. DOI: 10.7671/j.issn.1001-411X.2016.01.012
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To set up a method for rapid detection of different provenances, to investigate the genetic diversity, and to provide a theoretical basis for development, utilization and selective breeding of Melia azedarach.
Twenty SRAP primer combinations (PCs) with relatively high polymorphisms from 783 PCs were selected to analyze the genetic diversity of 37 provenances from all distribution areas nationwide and 1 provenance from Kenya.
The results showed 242 clear bands were amplified by 20 PCs, among which 101 bands (40.89%) were polymorphic. The number of bands of each PC ranged from 5 to 17, with an average of 12.1. The polymorphism information content (PIC) values of these PCs ranged from 0.188 to 0.488, with an average of 0.299. Thirty-eight provenances could be divided into 7 categories based on UPGMA (with a threshold value of 0.350), and furthermore, by excluding 2 provenances, the rest 36 provenances could be divided into 5 geographic groups.
SRAP markers can be used to effectively evaluate the genetic diversity of Melia azedarach, and the results of cluster analysis demonstrate clear geographical trends and climatic-ecological characteristics.
| [1] |
杨湘. 不同果核群苦楝中苦楝素含量变化规律研究[D]. 南京: 南京林业大学, 2011.
|
| [2] |
刘丽云.苦楝栽培技术及应用价值[J].北方园艺, 2009(9):144-145. http://d.old.wanfangdata.com.cn/Periodical/bfyany200909054
|
| [3] |
曾东东. 家具用苦楝集成材的研究[D]. 南京: 南京林业大学, 2011.
|
| [4] |
程诗明, 顾万春.苦楝遗传资源学研究进展及其展望[J].浙江林业科技, 2007, 27(2):64-69. doi: 10.3969/j.issn.1001-3776.2007.02.017
|
| [5] |
单雪, 王秀利, 仇雪梅.分子标记及其在海洋动物遗传研究中的应用[J].生物技术通讯, 2005, 16(4): 46. http://d.old.wanfangdata.com.cn/Periodical/swjstx200504034
|
| [6] |
姚红伟, 张立冬, 孙金阳, 等. DNA分子标记技术概述[J].河北渔业, 2010(7): 42-46. doi: 10.3969/j.issn.1004-6755.2010.07.020
|
| [7] |
程诗明. 苦楝聚合群体遗传多样性研究与核心种质构建[D]. 北京: 中国林业科学研究院, 2005.
|
| [8] |
陈羡德. 植物源农药原料林树种苦楝良种选育及其遗传多样性分析[D]. 福州: 福建农林大学, 2008.
|
| [9] |
夏海涛. 药用苦楝遗传多样性ISSR分析和遗传变异规律研究[D]. 福州: 福建农林大学, 2009.
|
| [10] |
董星光, 樊丽, 王志刚, 等.梨SRAP体系的正交优化研究[J].江苏农业科学, 2009(2):51-53. doi: 10.3969/j.issn.1002-1302.2009.02.016
|
| [11] |
张晓蕾, 张凯旋, 杨传平, 等.白桦SRAP反应体系的建立与优化[J].东北林业大学学报, 2010, 38(9):1-3. doi: 10.3969/j.issn.1000-5382.2010.09.001
|
| [12] |
令狐斌, 侯思宇, 孙朝霞, 等.苦荞SRAP分子标记体系优化与遗传多样性分析[J].中国农业大学学报, 2015, 20(1):37-43. http://d.old.wanfangdata.com.cn/Periodical/zgnydxxb201501005
|
| [13] |
XIE W G, ZHANG X Q.Genetic diversity analysis and transferability of cereal EST-SSR markers to orchardgrass (Dactylis glomerata L.) [J]. Biochem Syst Ecol, 2010, 38(4):740-749. doi: 10.1016/j.bse.2010.06.009
|
| [14] |
陈丽君, 邓小梅, 丁美美, 等.苦楝种源果核及种子性状地理变异的研究[J].北京林业大学学报, 2014, 36(1):15-20. http://d.old.wanfangdata.com.cn/Periodical/bjlydxxb201401003
|
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