Stability analysis of internal reference gene of <i>Ricinus communis</i> treated by glucose-fipronil

MAO Genlin; XIE Yun; ZHAO Junlong; CHEN Yan; XU Hanhong; LIN Fei

doi:10.7671/j.issn.1001-411X.2016.01.009

Journal of South China Agricultural University > 2016 > 37(1): 52-57. > DOI: 10.7671/j.issn.1001-411X.2016.01.009

MAO Genlin, XIE Yun, ZHAO Junlong, CHEN Yan, XU Hanhong, LIN Fei. Stability analysis of internal reference gene of Ricinus communis treated by glucose-fipronil[J]. Journal of South China Agricultural University, 2016, 37(1): 52-57. DOI: 10.7671/j.issn.1001-411X.2016.01.009

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Stability analysis of internal reference gene of Ricinus communis treated by glucose-fipronil

College of Agriculture, South China Agricultural University/Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642, China

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Received Date: April 01, 2015
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
To screen reliable internal reference genes of castor, Ricinus communis, treated by glucose-fipronil (GTF) and the solvent dimethyl sulfoxide (DMSO), and to provide a basis for studying the uptake mechanism of GTF.
Method
Actin, ARC, ef1a, SamDC and TUA6 genes were selected as candidate reference genes. Specific primers for each gene were designed and real-time quantitative PCR was conducted. Five softwares, including geNorm, NormFinder, BestKeeper, Delta CT and RefFinder, were employed to analyze the gene expression stabilities in cotyledon of castor seedlings treated by GTF and DMSO with different concentration and time.
Result
The stabilities were variant according to different softwares. The rank orders of stability were geNorm: Actin=ef1a >SamDC > ARC > TUA6; NormFinder:SamDC > ARC > Actin > ef1a > TUA6; BestKeeper: Actin > ef1a > SamDC > ARC > TUA6; Delta CT: Actin > ef1a > SamDC > ARC > TUA6; RefFinder: Actin > SamDC > ef1a > ARC > TUA6. With a single analysis of DMSO treatment by RefFinder showed that ef1a > SamDC > Actin > TUA6 > ARC.
Conclusion
Actin is the stablest reference gene under GTF and DMSO treatments, while ef1a is the stablest one under DMSO treatment.
- Ricinus communis,
- GTF,
- DMSO,
- RT-qPCR,
- internal reference gene,
- stability

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Stability analysis of internal reference gene of Ricinus communis treated by glucose-fipronil

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