A SYBR Green I real-time fluorescence quantitative PCR detection method for Cotton leaf curl virus

Objective Cotton Leaf Curl Multan Virus (CLCuMV) is one of the most important viralpathogens of Malvaceae plants. The purpose of this study is to establish an accurate detection method forCLCuMV, which can provide a technological means to study propagation dynamics of viruse in hostplants, virus generation cycle inside vector insect, and interaction mechanisms among virus, plant andvector.

Method  The SYBR Green I real-time fluorescence quantitative PCR was adopted usingCLCuMV-plasmid as template and the standard curve was established. In accordance with the standardcurve, the CLCuMV contents in leaves of Hibiscus rose-sinensis and vector bodies of Benisia tabaci werecalculated.

Result and conclusion  The detection sensitivity was as low as 5.2 × 10¹ μL^-1 of CLCuMV, which was 10 times as sensitive as conventional PCR. It can be applied to quantitatively detect CLCuMVin host plants and vector insects.