Effects of different phosphorus levels on proline biosynthesis and accumulation and key gene expression in sugarcane

YANG Shan; YE Changhui; MO Junjie; HUANG Xing; YANG Jiewen; ZHOU Hongkai

doi:10.7671/j.issn.1001-411X.2015.06.011

Journal of South China Agricultural University > 2015 > 36(6): 68-75. > DOI: 10.7671/j.issn.1001-411X.2015.06.011

YANG Shan, YE Changhui, MO Junjie, HUANG Xing, YANG Jiewen, ZHOU Hongkai. Effects of different phosphorus levels on proline biosynthesis and accumulation and key gene expression in sugarcane[J]. Journal of South China Agricultural University, 2015, 36(6): 68-75. DOI: 10.7671/j.issn.1001-411X.2015.06.011

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Effects of different phosphorus levels on proline biosynthesis and accumulation and key gene expression in sugarcane

College of Agriculture, Guangdong Ocean University, Zhanjiang 524088, China

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Received Date: March 22, 2015
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
Different phosphorus(P) fertilizer rates were applied to clarify the effect of P fertilizer on proline biosynthesis and accumulation and drought-resistance in sugarcane.
Method
Sugarcane variety YT-55 was used as the plant material for barrel experiment.Under the normal and drought conditions, four P levels were designed.The physiological and biochemical indexes such as the expressions of P5CS and δ-OAT genes, enzyme activities and the contents of free proline and chlorophyll were determined.
Result and conclusion
The expressions of P5CS and δ-OAT genes, enzyme activities and the free proline content were affected by the ratios of N, P and K fertilizers under the normal and drought conditions.When the P level was 900 kg·hm^-2, both P5CS and δ-OAT activities were relatively low, and the free proline content was the lowest in the plants under the normal water condition.The P5CS activity was the highest; the δ-OAT activity was also at a high level; and the free proline content was up to the maximum amount under drought stress.Based on the results, the optimum levels of N, P and K fertilizers are 918 kg·hm^-2urea, 900 kg·hm^-2calcium superphosphate and 750 kg·hm^-2potassium chloride in Zhanjiang lateritic soil.The results of correlation, partial correlation and path analysis indicated that the P5CS was more effective than δ-OAT in free proline biosynthesis and accumulation of sugarcane under drought stress.The main pathway of free proline biosynthesis and accumulation is glutamic acid pathway (Glu→Pro) supplemented by ornithine pathway (Orn→ Pro) under drought stress.
- sugarcane,
- phosphorus application rate,
- proline content,
- δ-OAT,
- P5CS,
- gene expression,
- drought stress

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References (37)

References

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Effects of different phosphorus levels on proline biosynthesis and accumulation and key gene expression in sugarcane

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