Prevalence of resistance to disinfectants and antibiotics in <i>Escherichia coli</i> isolated from the swine and farm environment

YANG Qiu'e; LI Liang; LIAO Xiaoping; LI Xingping; WANG Mingru; FANG Xi; LIU Yahong; SUN Jian

doi:10.7671/j.issn.1001-411X.2015.06.003

Journal of South China Agricultural University > 2015 > 36(6): 15-22. > DOI: 10.7671/j.issn.1001-411X.2015.06.003

YANG Qiu'e, LI Liang, LIAO Xiaoping, LI Xingping, WANG Mingru, FANG Xi, LIU Yahong, SUN Jian. Prevalence of resistance to disinfectants and antibiotics in Escherichia coli isolated from the swine and farm environment[J]. Journal of South China Agricultural University, 2015, 36(6): 15-22. DOI: 10.7671/j.issn.1001-411X.2015.06.003

Citation:

PDF (525 KB)

Prevalence of resistance to disinfectants and antibiotics in Escherichia coli isolated from the swine and farm environment

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

More Information

Received Date: January 19, 2015
Available Online: May 17, 2023

Graphical Abstract

Abstract

Abstract

Objective
The prevalence of disinfectant resistance genes and the relationship between resistance to disinfectants and antibiotics in Escherichia coli recovered from the swine and farm environment were investigated.
Method
A total of 130 samples collected from swine manure, air, pond water, soil and farm workers were screened for the presence of qacE, qacE△1, qacF, qacG, emrE, sugE(c), sugE(p), mdfA and ydgE/ydgF resistance genes. The susceptibilities of E. coli strains to four disinfectants and ten common antibiotics were determined using the agar dilution method.
Result and conclusion
Ninety-seven strains of E. coli were obtained from 130 samples with detection frequency of 74.6%. All E. coli isolates showed reduced susceptibility to four disinfectants. The minimum inhibitory concentrations (MICs) of benzalkonium chloride (BC) and cetyltrimethy ammonium bromide (CTAB) against E. coli were higher than those of triclosan (TCS) and chlorhexidine (CL). The percent of E. coli strains, which displayed multi-drug resistant phenotypes, was 87.6% (85/97), and the resistance rates of sulfamethoxazole/trimethoprim (S/T), doxycycline(DOX), nalidixic acid (NAL), cefotaxime (CTX) and florfenicol (FFC) were high(68%-88%). However, the disinfectant resistance genes were less prevalent (2.1%-20.6%), with the most prevalent of qacE△1 found in 97 E. coli strains. There was a positive correlation between antimicrobial resistance and high MICs of quaternary ammonium compounds. The close relationship between the detection rate of disinfectant resistance genes and six antimicrobials resistance in E. coli strains was found, which resisted S/T, NAL, ciprofloxacin (CIP), cefoxitin (FOX), AMK and CS, respectively. The co-resistance E. coli poses a challenge to environmental management. Therefore, regulation of using antibiotics and disinfectants is urgently needed in order to slow down the emergence of drug-resistant bacteria.
- Escherichia coli,
- disinfectant resistance,
- resistance rate,
- antimicrobial

FullText(HTML)

References (21)

References

[1]	HOLZEL C S, HARMS K S, BAUER J, et al.Diversity of antimicrobial resistance genes and class-1-integrons in phylogenetically related porcine and human Escherichia coli [J]. Vet Microbiol, 2012, 160(3 /4): 403-412. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=JJ0231377590
[2]	XIONG W, SUN Y, ZHANG T, et al.Antibiotics, antibiotic resistance genes, and bacterial community composition in fresh water aquaculture environment in China[J]. Microb Ecol, 2015, 70(2):425-432. doi: 10.1007/s00248-015-0583-x
[3]	MCDONNELL G, RUSSELI A D.Antiseptics and disinfectants: Activity, action, and resistance[J]. Clin Microbiol Rev, 1999, 12(1): 147-179. http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ026938130/
[4]	BJORLAND J, STEINUM T, KVITLE B, et al.Widespread distribution of disinfectant resistance genes among Staphylococci of bovine and caprine origin in Norway[J]. J Clin Microbiol, 2005, 43(9): 4363-4368. doi: 10.1128/JCM.43.9.4363-4368.2005
[5]	BAY D C, ROMMENS K L, TURNER R J.Small multidrug resistance proteins: A multidrug transporter family that continues to grow[J]. Biochim Biophys Acta, 2008, 1778(9): 1814-1838. doi: 10.1016/j.bbamem.2007.08.015
[6]	KANG H Y, JEONG Y S, OH J Y, et al.Characterization of antimicrobial resistance and class Ⅰ integrons found in Escherichia coli isolates from humans and animals in Korea[J]. J Antimicrob Chemother, 2005, 55 (5): 639-644. doi: 10.1093/jac/dki076
[7]	WELCH T J, FRICKE W F, McDERMOTT P F, et al. Multiple antimicrobial resistance in plague: An emerging public health risk[J /OL]. PLoS One, 2007, 2 (3): e309. [2015-01-10] http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0000309.
[8]	WELCH T J, EVEHUIS J, WHITE D G, et al.IncA /C plasmid-mediated florfenicol resistance in the catfish pathogen Edwardsiella ictaluri[J ]. Antimicrob Agents Chemother, 2009, 53(2): 845-846. doi: 10.1128/AAC.01312-08
[9]	BAY D C, TURNER R J.Diversity and evolution of the small multidrug resistance protein family[J]. BMC Evol Biol, 2009, 9: 140. doi: 10.1186/1471-2148-9-140
[10]	阮燕平.革兰氏阴性菌耐消毒剂基因的研究进展[J].中国消毒学杂志, 2012, 29(10): 915-917.
[11]	HAMER D H, GILL C J.From the farm to the kitchen table: The negative impact of antimicrobial use in animals on humans[J]. Nutr Rev, 2002, 60(8): 261-264. doi: 10.1301/002966402320289395
[12]	GILBERT P, MCBAIN A J.Potential impact of increased use of biocides in consumer products on prevalence of antibiotic resistance[J]. Clin Microbiol Rev, 2003, 16(2): 189-208. doi: 10.1128/CMR.16.2.189-208.2003
[13]	只帅, 席美丽, 刘攻关, 等.陕西部分地区不同食源性大肠埃希菌耐药性检测[J].中国食品学报, 2011, 11 (1): 196-201. doi: 10.3969/j.issn.1009-7848.2011.01.029
[14]	Clinical and laboratory standards institute. Performance standards for antimi-crobial disk and dilution susceptibility tests for bacteria isolated from animals: Documents VET01-A4E and VET01-S2E[S]. Wayne, PA, USA: CLSI, 2013.
[15]	BUFFET-BATAILLON S, BRANGER B, CORMIER M, et al.Effect of higher minimum inhibitory concentrations of quaternary ammonium compounds in clinical E.coli isolates on antibiotic susceptibilities and clinical outcomes [J]. J Hosp Infect, 2011, 79(2): 141-146. doi: 10.1016/j.jhin.2011.06.008
[16]	HUETA A, RAYGADA J L, MENDIRATTA K, et al. Multidrug efflux pump over expression in Staphylococcus aureus after single and multiple in vitro exposures to biocides and dyes[J]. Microbiol, 2008, 154 (10): 31443153. doi: 10.1099/mic.0.2008/021188-0
[17]	FUENTES D E, NAVARRO C A, TANTALEAN J C, et al.The product of the qacC gene of Staphylococcus epider-midis CH mediates resistance to beta-lactam antibiotics in gram-positive and gram-negative bacteria[J]. Res Microbiol, 2005, 156(4): 472-477. doi: 10.1016/j.resmic.2005.01.002
[18]	SCHLUTER A, SZCZEPANOWSKI R, PUHLER A, et al.Genomics of IncP-1 antibiotic resistance plasmids isolated from waste water treatment plants provide evidence for a widely accessible drug resistance gene pool[J ]. FEMS Microbiol Rev, 2007, 31(4): 449-477. doi: 10.1111/j.1574-6976.2007.00074.x
[19]	费春楠, 刘军, 沈凡, 等.医院内感染病原菌对常见消毒剂耐药性的研究[J].环境与健康杂志, 2007, 24(12): 1011-1012. doi: 10.3969/j.issn.1001-5914.2007.12.033
[20]	林辉, 郑剑.铜绿假单胞菌对消毒剂抗药性的研究进展[J].中国消毒学杂志, 2007, 24(3): 269-271. doi: 10.3969/j.issn.1001-7658.2007.03.027
[21]	SOUMET C, FOURREAU E, LEGRANDOIS P, et al. Resistance to phenicol compounds followingadaptation to quaternary ammonium compounds in Escherichia coli[J]. Vet Microbiol, 2012, 158 (1 /2): 147-152.

Cited By

Get Citation

PDF

XML

Article views (1744) PDF downloads (2116)

Turn off MathJax

Article Contents

Abstract

References

Prevalence of resistance to disinfectants and antibiotics in Escherichia coli isolated from the swine and farm environment

Abstract

References

Catalog

Export File

Citation

Format

Content