Molecular identification and inheritance analysis of transgenic pigs produced by incubation of sperm and lentivirus

Objective This study investigated the inheritance analysis of foreign genes in transgenic pigs produced previously by using incubation of sperm and lentivirus.

Method Transgenic pigs were reproduced by mating with wildtype pigs, and their offsprings of transgenic G₁ and G₂ were examined for inher-itance of foreign gene with PCR and Southern-blot methods.

Result and conclusion The results showed that PCR-positive rate was 25.42%(15/59)and 46.67%(7/15)in G₁ and G₂ respectively.In the 13 tissues of the 12 positive transgenic pigs, the foreign genes were found in many tissues; RT-PCR detection showed 66.67%(24/36)tissue samples from stomach, pituitary and hypothalamus of positive pigs represented foreign gene expression.The results indicate foreign genes of G₀ pigs produced by incubation of sperm and lentivirus can be integrated in the pig genome and transmitted to their offsprings.