Development and application of indirect ELISA for porcine epidemic diarrhea virus antibody detection

Objective Two types of ELISA methods for porcine epidemic diarrhea virus (PEDV) antibody detection were established.

Method The inactivated PEDV strain GD-1 and expressed COE protein as coating antigen were used to establish optimal ELISA conditions and test the repeatability, specificity and clinical application effectiveness.

Result and conclusion The optimal conditions of two types of ELISA were established.The variation coefficient of two EILSA methods was less than 4. 60% and 5. 30% re spectively.The specific test results of foot and mouth disease virus(FMDV), classical swine fever virus (CSFV), porcine circovirus(PCV), porcine reproductive and respiratory syndrome virus(PRRSV) posi tive serum were all negative.The total positive rate of 223 clinical serum samples was 97. 3% (217 /223) and 98. 6% (220 /223) respectively.These two methods have a good stability and long shelf life.This research proves that two types of ELISA methods have a good detection rate and a high coincidence rate which will provide a technical method for PEDV antibody detection and epidemiological investigation.