Improved methods to recover and reamplify trace DNA fragments from the polymorphic PAGE band

Objective To optimize the methods of recovery and reamplification of trace DNA fragments from polymorphic polyacrylamide gel electrophoresis (PAGE) bands.

Method Effects of four methods of dissolving polyacrylamide gel and recovery efficiencies of trace nucleic acids were obtained.

Result and conclusion Dissolving/Bing buffer from Bioteke kit showed high recovery rates of DNA fragments from PAGE gels with only 1.0-7.5 ng nucleotide. Effects of amplification positively correlated with recovery efficiencies. The separation of liquid mixture and impurities was the key to successful amplification. This research forms a fast, efficient DNA recycling and amplification method from polymorphism PAGE, which provides a new approach to analyze the causes of genetic diversity.