In vitro propagation of Acacia mangium×A. auriculiformis

Objective To improve the propagation efficiency and extend the planting of Acacia mangium×A. auriculiformis.

Method This study was carried out to explore the techniques of in vitro propagation of A.mangium×A. auriculiformis using stem segments with buds collected from 16-year plants as the explant.

Result and conclusion Current season stem segments carrying 3-5 axillary buds were sterilized with w=0.1% mercuric chloride and φ＝75% alcoho1 for 15 min and 30 s respectively before they were inoculated onto MS medium. Buds could be induced successfully on MS with a shooting rate of 95.68%. The bud proliferation index was 3.97 after being transferred onto MS+6-BA 1.5 mg·L^-1+NAA 0.1 mg·L^-1+ sucrose 30-40 g·L^-1 for 35 d. The buds all rooted on the 15^th day from inoculation onto 1/2 MS medium after pre-cultured on MS supplemented with IBA 600 mg·L^-1 for 4-8 h. Inoculating the proliferated buds onto 1/2 MS+IBA 1.0 mg·L^-1+NAA 0.5 mg·L^-1+sucrose 30 g·L^-1 also generated a high rooting rate of 99.43%. The survival rate reach 94.67% after the rooted plantlet are transplanted to the yellow soil medium.