Effects of metal resistant plant growth promoting bacteria on corn growth and Zn accumulation

TANG Fei; CHEN Yagang; XU Huan; LONG Xinxian

doi:10.7671/j.issn.1001-411X.2015.02.005

Journal of South China Agricultural University > 2015 > 36(2): 25-30, 68. > DOI: 10.7671/j.issn.1001-411X.2015.02.005

TANG Fei, CHEN Yagang, XU Huan, LONG Xinxian. Effects of metal resistant plant growth promoting bacteria on corn growth and Zn accumulation[J]. Journal of South China Agricultural University, 2015, 36(2): 25-30, 68. DOI: 10.7671/j.issn.1001-411X.2015.02.005

Citation:

PDF (804 KB)

Effects of metal resistant plant growth promoting bacteria on corn growth and Zn accumulation

College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China

More Information

Received Date: December 08, 2013
Available Online: May 17, 2023

Abstract

Abstract

Objective
Effects of metal resistant plant growth promoting bacteria(PGPB) on corn growth and Zn accumulation were studied to provide a fundamental groundwork for mitigating the stress of heavy metal on crop growth and decreasing heavy metal uptake.
Method
A pot experiment was carried out to study the effects of inoculation with two Zn resistant PGPB, Ⅱ₂R₃ and Ⅳ₈R₃ on corn plant growth and Zn uptake from Zn contaminated soils (0, 200, 400 and 800 mg·kg^-1 Zn addition).
Result and conclusion
The strains Ⅱ₂R₃ and Ⅳ₈R₃ not only grew well with different concentrations of Zn, but also absorbed or accumulated much higher Zn from the growing medium. Individual inoculation of strain Ⅳ₈R₃ to the soil with 200 or 400 mg·kg^-1 Zn addition both promoted the corn growth. For the soils with 800 mg·kg^-1Zn addition, simultaneous inoculation with strains Ⅱ₂R₃ and Ⅳ₈R₃ promoted the corn growth. Individual or simultaneous inoculation with strains Ⅱ₂R₃ and Ⅳ₈R₃ to the soils at three Zn pollution levels (200, 400 and 800 mg·kg^-1 Zn addition) decreased the soil bioavailable Zn concentration. For the soil with a high Zn pollution level (800 mg·kg^-1Zn addition), the inoculation of strains Ⅱ₂R₃ orⅡ₂R₃+Ⅳ₈R₃ decreased Zn uptake and transportation in corn. However, all of three inoculation treatments had no significant effects on Zn uptake of corn when strains Ⅱ₂R₃, Ⅳ₈R₃, Ⅱ₂R₃+Ⅳ₈R₃ were inoculated to the soils with 200 or 400 mg·kg^-1 Zn addition. The results indicate that inoculation with metal resistant PGPB to high Zn-contaminated soil can promote the corn growth and inhibite the uptake and transportation of Zn in corn. Inoculation with strains Ⅱ₂R₃ or Ⅳ₈R₃ to high Zn-contaminated soils can inhibite the uptake and transportation of Zn in corn. The effects of inoculation with strains Ⅱ₂R₃ or Ⅳ₈R₃ on corn growth are related to the different levels of Zn pollution and characterization of the strains.
- Zinc pollution,
- metal resistant plant growth promoting bacterium,
- corn growth,
- Zn accumulation,
- bioavailability

FullText(HTML)

References (27)

References

[1]	杜锁军, 戴艳, 谢东俊, 等.土壤Zn污染及其治理措施评述[J].污染防治技术, 2009, 22(3): 87-90. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK200901745170
[2]	禇天铎, 刘新保.锌素营养对作物叶片解剖结构的影响[J].植物营养与肥料学报, 1995, 1(1): 24-29. doi: 10.3321/j.issn:1008-505X.1995.01.004
[3]	ZHAO F J, LOMBI E, MCGRATH S P. Assessing the potential for zinc and cadmium phytore-mediation with the hyperaccumulator Thlaspi caerulescens[J]. Plant and Soil, 2003(249): 37-43.
[4]	LOCK K, JANSSEN C R. Influence of soil zinc concentrations on zinc sensitivity and functional diversity of microbial communities[J]. Environ Pollut, 2005(136): 275-281. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=JJ029130468
[5]	顾继光, 林秋奇, 胡韧, 等.矿区重金属在土壤-作物系统迁移行为的研究[J].农业环境科学学报, 2005, 24(4): 634-637. doi: 10.3321/j.issn:1672-2043.2005.04.003
[6]	祖艳群, 李元, 陈海燕, 等.蔬菜中铅镉铜锌含量的影响因素研究[J].农业环境科学学报, 2003, 22(3): 289-292. doi: 10.3321/j.issn:1672-2043.2003.03.009
[7]	CHEN X T, WANG G, LIANG Z C. Effect of amendments on growth and element uptake of pakchoi in a cadmium, zinc and lead contaminated soil[J]. Pedosphere, 2002, 12(31): 243-250. http://cn.bing.com/academic/profile?id=ab68a447a8f7cf2bbd9d886ff2347118&encoded=0&v=paper_preview&mkt=zh-cn
[8]	TABAK H H, LENS P, ERIC D, et al. Developments in bioremediation of soils and sediments polluted with metals and radionuclides :1:Microbial processes and mechanisms affecting bioremediation of metal contamination and influencing metal toxicity and transport[J]. Rev Environ Sci Bio/Technol, 2005, 4(3): 115-156. doi: 10.1007/s11157-005-2169-4
[9]	NIES D H, SILVER S. Bacterial transition metal homeostasis[J]. Mol Microbiol Heavy Metals, 2007, 6: 117-142. doi: 10.1007/978-3-540-69771-8
[10]	MEJÁRE M, BIILOW L. Metal-binding proteins and petides in bioremediation of heavy metal [J]. Trends Biotechnol, 2001, 19(2): 67-73. doi: 10.1016/S0167-7799(00)01534-1
[11]	LUO S L, WAN Y, XIAO X, et al. Isolation and characterization of endophytic bacterium LRE07 from cadmium hyperaccumulator Solanum nigrum L. and its potential for remediation[J]. Appl Microbiol Biotechnol, 2011(89): 1637-1644. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=JJ0224623827
[12]	MACASKIE L E, DEAN A C R, CHEETHAN A K, et al. Cadmium accumulation by a Citrobacter sp.: The chemical nature of the accumulated metal precipitate and its location on the bacterial cells[J]. J Gen Microl, 1987(133): 539-544.
[13]	马莹, 骆永明, 腾应, 等.内生细菌强化重金属污染土壤植物修复研究进展[J].土壤学报, 2013, 50(1): 195-202. http://d.old.wanfangdata.com.cn/Periodical/trxb201301024
[14]	LONG X X, CHEN X M, CHEN Y G, et al. Isolation and characterization endophytic bacteria from hyperaccumulator Sedum alfredii Hance and their potential to promote phytoextraction of zinc polluted soil[J]. World J Microbiol Biotechnol, 2011, 27(5): 1197-1207. doi: 10.1007/s11274-010-0568-3
[15]	陈雪梅. 东南景天内生细菌的分离及其强化植物修复锌污染土壤的研究[D]. 广州: 华南农业大学, 2010. http://www.wanfangdata.com.cn/details/detail.do?_type=degree&id=Y1765598
[16]	郭晓方, 卫泽斌, 丘锦荣, 等.玉米对重金属累积与转运的品种间差异[J].生态与农村环境学报, 2010, 26 (4): 367-371. doi: 10.3969/j.issn.1673-4831.2010.04.015
[17]	鲍士旦.土壤农化分析[M].北京:中国农业出版社, 2000.
[18]	魏威, 梁东丽, 陈世宝.土壤中外源锌对不同植物毒性的敏感性分布[J].生态学杂志, 2012, 31(3): 538-543. http://d.old.wanfangdata.com.cn/Periodical/stxzz201203005
[19]	刘春早, 黄益宗, 雷鸣, 等.湘江流域土壤重金属污染及其生态环境风险评价[J].环境科学, 2012, 33(1): 263-268. http://d.old.wanfangdata.com.cn/Periodical/hjkx201201043
[20]	刘春早, 黄益宗, 雷鸣, 等.重金属污染评价方法(TCLP)评价资江流域土壤重金属生态风险[J].环境化学, 2011, 30(9): 1582-1589. http://d.old.wanfangdata.com.cn/Periodical/hjhx201109010
[21]	朱桂芬, 张春燕, 王建玲, 等.新乡市寺庄顶污灌区土壤及小麦重金属污染特征的研究[J].农业环境科学学报, 2009, 28: 263-268. doi: 10.3321/j.issn:1672-2043.2009.02.008
[22]	MA Y, PRASAD M N V, RAJKUMAR M, et al. Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soils[J]. Biotechnol Adv, 2011, 29(2): 248-258. doi: 10.1016/j.biotechadv.2010.12.001
[23]	钱春香, 王明明, 许燕波.土壤重金属污染现状及微生物修复技术研究进展[J].东南大学学报, 2013, 43(3): 669-674. http://d.old.wanfangdata.com.cn/Periodical/dndxxb201303041
[24]	PAL A, PAUL A K. Microbial extracellular polymeric substances: Central elements in heavy metal bioremediation [J]. India J Microbial, 2008(99): 536-548. http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_3450203
[25]	MADHAIYAN M, POONGUZHALI S, SA T. Metal tolerating methylotrophic bacteria reduces nickel and cadmium toxicity and promotes plant growth of tomato (Lycopersicon esculentum L.)[J]. Chemosphere, 2007, 69(2): 220-228. doi: 10.1016/j.chemosphere.2007.04.017
[26]	VIVAS A, BIRO B, RUIZ-LOZANO J M, et al. Two bacterial strains isolated from a Zn-polluted soil enhance plant growth and mycorrhizal efficiency under Zn-toxicity[J]. Chemosphere, 2006, 62(9): 1523-1533. doi: 10.1016/j.chemosphere.2005.06.053
[27]	FENG M H, SHAN X Q, ZHANG S Z, et al. A comparison of the rhizosphere-based method with DTPA, EDTA, CaCl₂ and NaNO₃ extraction methods for prediction of bioavailability of metals in soils to barley[J]. Environ Pollut, 2005(137): 231-240. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=JJ0210804484

Cited By

Corresponding author: LONG Xinxian, longxx@scau.edu.cn

College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China

Get Citation

PDF

XML

Article views (1778) PDF downloads (1266)

Turn off MathJax

Article Contents

Abstract

References

Effects of metal resistant plant growth promoting bacteria on corn growth and Zn accumulation

Abstract

References

Catalog

Corresponding author: LONG Xinxian, longxx@scau.edu.cn

Export File

Citation

Format

Content