Molecular cloning, expression and bioinformatic analysis of TaLEA2 gene from wheat

【Objective】The cloning, expression and bioinformatic analysis of TaLEA2 gene were carried out to lay the theoretical basis for salt-tolerance of wheat.【Method】The TaLEA2 gene was obtained by homology cloning. Its gene structures and protein characteristics were analyzed by bioinformatic softwares. Real-time PCR was used to analyze its expression.【Result and conclusion】The results showed that TaLEA2 contained a 981 bp ORF encoding 326 amino acids. Using bioinformatics analysis to predict and analyze TaLEA2, the protein had classic domains and characteristics of LEA2, rich in Lys and lack of Cys, high hydrophilia (GRAVY, -0.405) and heat stability (Instability index, 25.28). The results of real-time PCR revealed that the expression of TaLEA2 had a constitutive expression, whose levels were different in different tissues. The expression was induced markedly by drought, salt, low-temperature and pathogenic bacteria in particular, but no change in treatment with ABA. Therefore，it is speculated that TaLEA2 can play a role not only in growth and development conditions but also in stress stage of wheat, especially in resistance to drought-stress.