LI Weicheng, JI Changyun, ZHANG Jingjie, CHENG Hankui, DENG Chao, ZHONG Jialian, DENG Xianbai, SHEN Xiangguang. An optimization study on determination of fluoroquinolones residues in chicken muscle by high performance liquid chromatography[J]. Journal of South China Agricultural University, 2014, 35(5): 14-18. DOI: 10.7671/j.issn.1001-411X.2014.05.003
Citation:
LI Weicheng, JI Changyun, ZHANG Jingjie, CHENG Hankui, DENG Chao, ZHONG Jialian, DENG Xianbai, SHEN Xiangguang. An optimization study on determination of fluoroquinolones residues in chicken muscle by high performance liquid chromatography[J]. Journal of South China Agricultural University, 2014, 35(5): 14-18. DOI: 10.7671/j.issn.1001-411X.2014.05.003
LI Weicheng, JI Changyun, ZHANG Jingjie, CHENG Hankui, DENG Chao, ZHONG Jialian, DENG Xianbai, SHEN Xiangguang. An optimization study on determination of fluoroquinolones residues in chicken muscle by high performance liquid chromatography[J]. Journal of South China Agricultural University, 2014, 35(5): 14-18. DOI: 10.7671/j.issn.1001-411X.2014.05.003
Citation:
LI Weicheng, JI Changyun, ZHANG Jingjie, CHENG Hankui, DENG Chao, ZHONG Jialian, DENG Xianbai, SHEN Xiangguang. An optimization study on determination of fluoroquinolones residues in chicken muscle by high performance liquid chromatography[J]. Journal of South China Agricultural University, 2014, 35(5): 14-18. DOI: 10.7671/j.issn.1001-411X.2014.05.003
【Objective】A method for rapid determination of five fluoroquinolones (norfloxacin,ciprofloxacin,danofloxacin,enrofloxacin,sarafloxacin) residues in chicken muscle using high performance liquid chromatography (HPLC) was established.【Method】The extracting solution of the chicken samples included a mixture of acetonitrile and trichloroacetic acid(the mass fraction of 2%). HPLC mobile phase consisted of acetonitrile and phosphate buffer (0.02 mol·L-1) with triethy lamine. Fluorometric excitation wavelength was 280 nm, and emission wavelength was 450 nm. 【Result and conclusion】The recoveries of 5 fluoroquinolones were 84.71%-99.66% at three spiked level(low, medium, high), and the relative standard deviations (RSD) were 0.20%-4.34%.The limit of quantiation (LOQ) of norfloxacin, ciprofloxacin and enrofloxacin was 10 μg·kg-1. The LOQ of danofloxacin was 2 μg·kg-1, and the sarafloxacin was 20 μg·kg-1. The method is simple, practical, and sensitive. It can meet the needs of determination of fluoroquinolones in chicken muscle.