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    CHU Zhizhan, WU Guanghong, LIU Xiaolin. A research on extraction and enzymatic hydrolysis of zein[J]. Journal of South China Agricultural University, 2014, 35(3): 86-89. DOI: 10.7671/j.issn.1001-411X.2014.03.016
    Citation: CHU Zhizhan, WU Guanghong, LIU Xiaolin. A research on extraction and enzymatic hydrolysis of zein[J]. Journal of South China Agricultural University, 2014, 35(3): 86-89. DOI: 10.7671/j.issn.1001-411X.2014.03.016
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    A research on extraction and enzymatic hydrolysis of zein

    • 1 College of Life Sciences/Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms，South China Agricultural University,

    • 2 Yichun University,

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    • Received Date: September 21, 2013
      Abstract
    • 【Objective】The production of short peptide from zein was investigated to greatly promote the application of zein.【Method】The conditions such as extraction time, temperature, ethanol concentration and ratio of liquid to solid were optimized by orthogonal test. Zein was hydrolyzed by protease K and alkaline protease in this study.【Result and conclusion】The optimal conditions of extracting zein were as follows: the ratio of solid to liquid was 1∶4; the extracting time was 3 hours; the extracting temperature was 60 ℃ and the concentration of ethanol was 75%. Results showed that the zein could be hydrolyzed more efficiently by combining two enzymes than by only using one of the two enzymes. Zein hydrolyzed by protease K and alkaline protease in turn is the best enzymatic hydrolysis method.
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      LIU Xiaolin

      • 2 Yichun University,

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