Synthesis of artificial antigens and preparation for specific antisera against 3-amino-2-oxazolidinone，a metabolite of furaltadone

【Objective】To establish a high sensitivity enzyme-linked immunosorbent assay for the detection of furaltadone.【Method】Furaltadone metabolite (3-amino-5-morpholinomethyl-2-oxazolidinone, AMOZ) was coupled to carrier proteins (bovine serum albumin or ovalbumin) and reduced with NaBH₄ for the production of immunogen AMOZ-BSA and coating antigen AMOZ-OVA through glutaraldehyde method. With 4-carboxybenzaldehyde for derivatization reagent, AMOZ was made into CPAMOZ, which was successfully conjugated to carrier proteins according to the active ester method to form CPAMOZ-BSA, CPAMOZ-OVA. UV scanning showed that antigens were successfully linked to carrier proteins. After immunizing animal (Balb/c mice), polyclonal antibody against NPAMOZ was produced.【Result and conclusion】 Antibody was diluted at 1∶32×10⁴ and the IC₅₀ value was 4.53 ng·mL^-1. The cross-reactivity (CR) of the antibody with CPAMOZ, AMOZ and furaltadone was 78.6%, 1.5% and 4.6% respectively. There is almost no CR with other three nitrofurans and their metabolites, which indicates a high selectivity of the antibody.