Synthesis of a newly designed artificial antigen and preparation of a monoclonal antibody against salbutamol

【Objective】 To prove that the artificial antigen which was synthesised by 4-bromine ethyl butyrate was successful，and that the monoclonal antibody had a high sensitivity and specificity.【Method】 Salbutamol sulfate was connect with 4-bromine ethyl butyrate，then converted into hapten（SAL-HS）.The immunogen SAL-BSA(salbutamol-bovine serum albumin) and coating antigen SAL-OVA（ovalbumin）were synthesized by the N-Hydroxy-succinimide active ester method. The conjugations were identified by SDS-PAGE,UV and mass spectrum.Balb/c mice were immunized by immunogen; splenocytes were fused with SP2/0 myeloma cells; hybridomas cells were produced for a monoclonal antibody by ascites in vivo to identify immunological characteristics.【Result and conclusion】 Cultural supernatants titer of hybridomas cells and ascites were 1∶128 000 and 1∶2 560 000 respectively; the subclasses of monoclonal antibody was IGg1; the IC₅₀ of standard curve was 0.746 ng/mL, with other drugs of similar structure showing cross reactivity affinities being less than 0.015%.