ZHANG Ying, LIU Xiang-yin, LUO Yong-wen, YANG Xian-feng, HUANG Yong-liang, SHI He-he, FU Qiu-ling, YANG You-tian, WU Xiao-wei, GUO Xiao-feng. Analysis of N and G Gene of Rabies Virus Isolated from Pig[J]. Journal of South China Agricultural University, 2012, 33(4): 544-549. DOI: 10.7671/j.issn.1001-411X.2012.04.023
    Citation: ZHANG Ying, LIU Xiang-yin, LUO Yong-wen, YANG Xian-feng, HUANG Yong-liang, SHI He-he, FU Qiu-ling, YANG You-tian, WU Xiao-wei, GUO Xiao-feng. Analysis of N and G Gene of Rabies Virus Isolated from Pig[J]. Journal of South China Agricultural University, 2012, 33(4): 544-549. DOI: 10.7671/j.issn.1001-411X.2012.04.023

    Analysis of N and G Gene of Rabies Virus Isolated from Pig

    • To understand the difference of the nucleoprotein (N) gene and glycoprotein (G) gene between the strain of pig origin and other rabies viruses. The N and G genes of rabies virus isolated from the brain of a rabid pig in Guangdong Province were amplified by RT-PCR, cloned, sequenced and compared with the genes of other rabies virus. The results showed that the sequence of nucleotides and amino acids of N genes of GD-SH01 shared 84.3%-98.0% and 92.5%-99.3% similarity,and G genes of GD-SH01 shared 80.4%-98.2% and 87.8%-99.6% similarity, respectively, compared to other viruses.
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