Construction and Expression of Protokaryon Expression Plasmid for H and F Genes of Canine Distemper Virus Isolated from Mink

H and F genes were obtained by digesting the cloning plasmid pMD-18T-H and pMD-18T-F respectively.The recombinant protokaryon expression plasmids pET28a-H and pET28a-F were constructed by sub-cloning the objective gene into pET28a (+) vector. Recombinant protokaryon expression plasmids pET28a-H and pET28a-F were transformed into Escherichia coli Rosetta2(DE3)cell. Target genes were successfully expressed by SDS-PAGE detection. The size of the recombinant fusion proteins was 31 400 (H) and 38 200 (F) respectively. The recombinant fusion proteins showed positive reaction to anti-CDV canine serum by Western-blot detection.