Construction of a Recombinant Baculovirus Surface Displaying S1 Protein of M41 Strain of Infectious Bronchitis Virus

The S1（Spike）protein gene without membrane localization signal of infectious bronchitis virus (dS1）was amplified from infectious bronchitis virus(M41) by PCR and sub-cloned into pBACsurf-1. The fusion gene containing S1 and gp64 was then inserted into baculovirus transfer vector pFastBac^TM Dual to construct the recombinant plasmid pFastBac-gp64-dS1.Then,the plasmid was transformed into Escherichia coli DH10Bac competent cells to obtain the recombinant shuttle vector Bacmid-gp64-dS1. Extracted Bacmid-gp64-dS1 was transfected into Sf9 cells to produce the recombinant baculovirus BV-dS1 by using Lipofectamine^TM 2000. Indirect immunofluorescent assay indicated that the recombinant baculovirus BV-dS1 could express S1 protein on infected Sf9 cell membrane.