Detection of PRRSV Variant Strain in Boar Semen and Sequence Analysis of NSP2 and ORF5 Genes

Porcine reproductive and respiratory syndrome virus(PRRSV) variant strains(NSP2 1 594-1 680 deletion) were identified by real-time RT-PCR from boar semen of a pig farm.cDNA of NSP2 and ORF5 genes of one strain named GDTS were cloned and sequenced.The results showed that NSP2 gene was composed of 950 amino acids,which had 1 amino acid deletion at the positions of 481 and 29 amino acids deletion at the positions of 532-560 compared with that of CH-1a and VR-2332,which had the same deletion compared with that ...