Sequence Analysis of 16S Ribosomal DNA of Phytoplasma Associated with Rice Orange Leaf

One set of the conserved primers was designed to amplify a 1 853 bp fragment which included 16S rDNA(1 527 bp),adjacent spacer sequence(234 bp) and partial sequence of 23S rDNA(92 bp) by PCR from total DNA sample extracted from diseased rice as the amplified template.Nucleotide sequencing of the amplified fragment indicated that rice orange leaf phytoplasma shared nucleotide similarity about 88% to 95% with other phytoplasmas from data of GenBank,and a maximum similarity at 95% with America aster yellow phy...