Prokaryotic Expression and Bioinformatics Analysis of the Nucleprotein Genes of Vesicular Stomatitis Virus

The nucleoprotein genes of vesicular stomatitis virus(VSV,Indiana type and New Jersey type) were amplified by reverse transcription polymerase chain reaction(RT-PCR),and then cloned into pMD20-T vector for sequencing and bioinformatics analysis.These genes were inserted into the prokaryotic expression plasmids pBCX.The recombinant plasmid VSV-IN-pBCX and VSV-NJ-pBCX were transformed into Escherichia.coli BL21(DE3) cells.The relative molecular mass of the recombinant protein was 82 000,which was detected by SDS-PAGE and reacted with VS positive serum in Western-blot.The recombinant nucleoproteins can be used as the specific diagnosis antigens for immunoassay such as ELISA.